Frontiers in Cellular and Infection Microbiology (Jan 2023)
Naturally acquired antibodies to gametocyte antigens are associated with reduced transmission of Plasmodium vivax gametocytes to Anopheles arabiensis mosquitoes
- Surafel K. Tebeje,
- Surafel K. Tebeje,
- Wakweya Chali,
- Wakweya Chali,
- Elifaged Hailemeskel,
- Elifaged Hailemeskel,
- Elifaged Hailemeskel,
- Jordache Ramjith,
- Abrham Gashaw,
- Temesgen Ashine,
- Desalegn Nebret,
- Endashaw Esayas,
- Tadele Emiru,
- Tizita Tsegaye,
- Karina Teelen,
- Kjerstin Lanke,
- Eizo Takashima,
- Takafumi Tsuboi,
- Nichole D. Salinas,
- Niraj H. Tolia,
- David Narum,
- Chris Drakeley,
- Benoit Witkowski,
- Amelie Vantaux,
- Matthijs M. Jore,
- William J. R. Stone,
- Ivo S. Hansen,
- Fitsum G. Tadesse,
- Fitsum G. Tadesse,
- Teun Bousema,
- Teun Bousema
Affiliations
- Surafel K. Tebeje
- Armauer Hansen Research Institute, Malaria and Neglected Tropical Disease Directorate, Addis Ababa, Ethiopia
- Surafel K. Tebeje
- Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, Netherlands
- Wakweya Chali
- Armauer Hansen Research Institute, Malaria and Neglected Tropical Disease Directorate, Addis Ababa, Ethiopia
- Wakweya Chali
- Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, Netherlands
- Elifaged Hailemeskel
- Armauer Hansen Research Institute, Malaria and Neglected Tropical Disease Directorate, Addis Ababa, Ethiopia
- Elifaged Hailemeskel
- Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, Netherlands
- Elifaged Hailemeskel
- Department of Biology, College of Natural and Computational Sciences, Wollo University, Dessie, Ethiopia
- Jordache Ramjith
- Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, Netherlands
- Abrham Gashaw
- Armauer Hansen Research Institute, Malaria and Neglected Tropical Disease Directorate, Addis Ababa, Ethiopia
- Temesgen Ashine
- Armauer Hansen Research Institute, Malaria and Neglected Tropical Disease Directorate, Addis Ababa, Ethiopia
- Desalegn Nebret
- Armauer Hansen Research Institute, Malaria and Neglected Tropical Disease Directorate, Addis Ababa, Ethiopia
- Endashaw Esayas
- Armauer Hansen Research Institute, Malaria and Neglected Tropical Disease Directorate, Addis Ababa, Ethiopia
- Tadele Emiru
- Armauer Hansen Research Institute, Malaria and Neglected Tropical Disease Directorate, Addis Ababa, Ethiopia
- Tizita Tsegaye
- Armauer Hansen Research Institute, Malaria and Neglected Tropical Disease Directorate, Addis Ababa, Ethiopia
- Karina Teelen
- Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, Netherlands
- Kjerstin Lanke
- Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, Netherlands
- Eizo Takashima
- Division of Malaria Research, Proteo-Science Center, Ehime University, Matsuyama, Ehime, Japan
- Takafumi Tsuboi
- Division of Malaria Research, Proteo-Science Center, Ehime University, Matsuyama, Ehime, Japan
- Nichole D. Salinas
- Laboratory of Malaria Immunology and Vaccinology, Division of Intramural Research, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, United States
- Niraj H. Tolia
- Laboratory of Malaria Immunology and Vaccinology, Division of Intramural Research, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, United States
- David Narum
- Laboratory of Malaria Immunology and Vaccinology, Division of Intramural Research, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, United States
- Chris Drakeley
- London School of Hygiene & Tropical Medicine, London, United Kingdom
- Benoit Witkowski
- Malaria Molecular Epidemiology Unit, Pasteur Institute of Cambodia, Phnom Penh, Cambodia
- Amelie Vantaux
- Malaria Molecular Epidemiology Unit, Pasteur Institute of Cambodia, Phnom Penh, Cambodia
- Matthijs M. Jore
- Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, Netherlands
- William J. R. Stone
- London School of Hygiene & Tropical Medicine, London, United Kingdom
- Ivo S. Hansen
- Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, Netherlands
- Fitsum G. Tadesse
- Armauer Hansen Research Institute, Malaria and Neglected Tropical Disease Directorate, Addis Ababa, Ethiopia
- Fitsum G. Tadesse
- Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, Netherlands
- Teun Bousema
- Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, Netherlands
- Teun Bousema
- London School of Hygiene & Tropical Medicine, London, United Kingdom
- DOI
- https://doi.org/10.3389/fcimb.2022.1106369
- Journal volume & issue
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Vol. 12
Abstract
Naturally acquired antibodies may reduce the transmission of Plasmodium gametocytes to mosquitoes. Here, we investigated associations between antibody prevalence and P. vivax infectivity to mosquitoes. A total of 368 microscopy confirmed P. vivax symptomatic patients were passively recruited from health centers in Ethiopia and supplemented with 56 observations from asymptomatic P. vivax parasite carriers. Direct membrane feeding assays (DMFA) were performed to assess mosquito infectivity; for selected feeds these experiments were also performed after replacing autologous plasma with malaria naïve control serum (n=61). The prevalence of antibodies against 6 sexual stage antigens (Pvs47, Pvs48/45, Pvs230, PvsHAP2, Pvs25 and PvCelTOS) and an array of asexual antigens was determined by ELISA and multiplexed bead-based assays. Gametocyte (ρ< 0.42; p = 0.0001) and parasite (ρ = 0.21; p = 0.0001) densities were positively associated with mosquito infection rates. Antibodies against Pvs47, Pvs230 and Pvs25 were associated with 23 and 34% reductions in mosquito infection rates (p<0.0001), respectively. Individuals who showed evidence of transmission blockade in serum-replacement DMFAs (n=8) were significantly more likely to have PvsHAP2 or Pvs47 antibodies. Further studies may demonstrate causality for the observed associations, improve our understanding of the natural transmission of P. vivax and support vaccine development.
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