MOLECULAR GENETIC ANALYSIS AND SPECTRUM OF MUTATIONS IN KATG, INHA, RPOB GENES CODING DRUG RESISTANCE TO ISONIAZID AND RIFAMPICIN IN THOSE SUFFERING FROM TB/HIV CO-INFECTION

Abstract

51 patients suffering from pulmonary tuberculosis with concurrent HIV infection (Group 1) had PCR testing in real time (ZAO Sintol, Moscow) of sputum samples in order to study the spectrum and prevalence of mutations in katG, inhA, rpoB genes coding drug resistance (DR) to isoniazid (H) and rifampicin (R). The control group included 70 HIV negative tuberculosis patients (Group 2). No significant differences in the number and spectrum of mutations in genes coding DR to H and R were found between two groups. However in Group 1 among clinical isolates with multiple drug resistance the confident prevalence of combination of mutations (rpoB Ser531- > Leu + katG Ser315- > Thr1) was found compared to Group 2 where the combined mutations of rpoB Ser531 > Leu + katG Ser315- > Thr1 + inhA 15 were registered more often where mutations in rpoB gene were combined with mutations simultaneously in two genes coding DR to H (katG + inhA).

Keywords

• туберкулез
• вич-инфекция
• множественная лекарственная устойчивость
• мутации в генах