Influence Of Intensive Exercise On Renal Functions (Rf) And Advanced Glycation End-Products (Ages)

Abstract

Under certain pathologic conditions AGEs formation can be increased beyond normal levels.The purpose of the study was to investigate essential RF and AGEs before, immediately after and 2 days after runs. Nine trained runners (43±9yr) during 9.5 km and 13 trained runners (48±11yr) during 16.3 km long-distance runs were investigated. Standard blood and urinary RF parameters were investi-gated in all runners using spectrophotometric methods by Roche analyzer Integra 800.Serum cystatin C was determined using immu-noturbidimetric method (PETIA Gentian). Plasma AGEs and malon-dialdehyde (MDA) were determined by spectrofluorimetric methods, Nε-carboxylmethyllysine using ELISA method and advanced oxidation protein products(AOPPs) using spectrophotometric method. Total proteinuria was 0.46±0.4 g/L after 9.5 km run and 0.35±0.3 g/L after 16.3 km run. Serum urea and creatinine significantly increased after both runs (creatinine in 28% after 9.5 km and in 41% after 16.3 km run). Estimated glomerular filtration rate (eGFR) MDRD and eGFR-PETIA significantly decreased after both runs (9.5 km: in 27.6% resp. 21.3% and 16.3 km: in 34,6% resp. 30.3%, p<0.01). Direct relationship between serum cystatin C and plasma AGEs after 16.3 km run was found (r=0.66, p=0,014). No significant changes in plasma AGEs (from 277±86—286±72 resp. from 283±64—292±90 AU), CML (from 619±78–665±131 resp. from 724±92—762±135 ng/mL) and AOPPs (from 151±58—106±21 resp. from 159±100–133±67 μmol/L) were found after both runs. Plasma MDA decreased after both runs. In conclusion, RF abnormalities in runners were caused by dehydration, protein catabolism, rhabdomyolysis and others. These RF changes were not present or parameters not significantly differed from initial values 2 days after both runs. Plasma AGEs and AOPPs in runners were in reference ranges, no significant changes during the both runs were observed.