Functional Access to Neuron Subclasses in Rodent and Primate Forebrain

Preeti Mehta; Lauren Kreeger; Dennis C. Wylie; Jagruti J. Pattadkal; Tara Lusignan; Matthew J. Davis; Gergely F. Turi; Wen-Ke Li; Matthew P. Whitmire; Yuzhi Chen; Bridget L. Kajs; Eyal Seidemann; Nicholas J. Priebe; Attila Losonczy; Boris V. Zemelman

Cell Reports (Mar 2019)

Functional Access to Neuron Subclasses in Rodent and Primate Forebrain

Preeti Mehta,
Lauren Kreeger,
Dennis C. Wylie,
Jagruti J. Pattadkal,
Tara Lusignan,
Matthew J. Davis,
Gergely F. Turi,
Wen-Ke Li,
Matthew P. Whitmire,
Yuzhi Chen,
Bridget L. Kajs,
Eyal Seidemann,
Nicholas J. Priebe,
Attila Losonczy,
Boris V. Zemelman

Affiliations

Preeti Mehta: Center for Learning and Memory, University of Texas, Austin, TX 78712, USA; Department of Neuroscience, University of Texas, Austin, TX 78712, USA
Lauren Kreeger: Center for Learning and Memory, University of Texas, Austin, TX 78712, USA; Department of Neuroscience, University of Texas, Austin, TX 78712, USA
Dennis C. Wylie: Center for Computational Biology and Bioinformatics, University of Texas, Austin, TX 78712, USA
Jagruti J. Pattadkal: Center for Learning and Memory, University of Texas, Austin, TX 78712, USA; Center for Perceptual Systems, University of Texas, Austin, TX 78712, USA; Department of Neuroscience, University of Texas, Austin, TX 78712, USA
Tara Lusignan: Center for Learning and Memory, University of Texas, Austin, TX 78712, USA; Department of Neuroscience, University of Texas, Austin, TX 78712, USA
Matthew J. Davis: Center for Learning and Memory, University of Texas, Austin, TX 78712, USA; Department of Neuroscience, University of Texas, Austin, TX 78712, USA
Gergely F. Turi: Kavli Institute for Brain Science, Columbia University, New York, NY 10027, USA; Department of Neuroscience, Columbia University, New York, NY 10027, USA
Wen-Ke Li: Kavli Institute for Brain Science, Columbia University, New York, NY 10027, USA; Department of Neuroscience, Columbia University, New York, NY 10027, USA
Matthew P. Whitmire: Center for Perceptual Systems, University of Texas, Austin, TX 78712, USA; Department of Neuroscience, University of Texas, Austin, TX 78712, USA; Department of Psychology, University of Texas, Austin, TX 78712, USA
Yuzhi Chen: Center for Perceptual Systems, University of Texas, Austin, TX 78712, USA; Department of Neuroscience, University of Texas, Austin, TX 78712, USA; Department of Psychology, University of Texas, Austin, TX 78712, USA
Bridget L. Kajs: Center for Learning and Memory, University of Texas, Austin, TX 78712, USA; Department of Neuroscience, University of Texas, Austin, TX 78712, USA
Eyal Seidemann: Center for Perceptual Systems, University of Texas, Austin, TX 78712, USA; Department of Neuroscience, University of Texas, Austin, TX 78712, USA; Department of Psychology, University of Texas, Austin, TX 78712, USA
Nicholas J. Priebe: Center for Learning and Memory, University of Texas, Austin, TX 78712, USA; Center for Perceptual Systems, University of Texas, Austin, TX 78712, USA; Department of Neuroscience, University of Texas, Austin, TX 78712, USA
Attila Losonczy: Kavli Institute for Brain Science, Columbia University, New York, NY 10027, USA; Department of Neuroscience, Columbia University, New York, NY 10027, USA
Boris V. Zemelman: Center for Learning and Memory, University of Texas, Austin, TX 78712, USA; Department of Neuroscience, University of Texas, Austin, TX 78712, USA; Corresponding author

Journal volume & issue: Vol. 26, no. 10
pp. 2818 – 2832.e8

Abstract

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Summary: Viral vectors enable foreign proteins to be expressed in brains of non-genetic species, including non-human primates. However, viruses targeting specific neuron classes have proved elusive. Here we describe viral promoters and strategies for accessing GABAergic interneurons and their molecularly defined subsets in the rodent and primate. Using a set intersection approach, which relies on two co-active promoters, we can restrict heterologous protein expression to cortical and hippocampal somatostatin-positive and parvalbumin-positive interneurons. With an orthogonal set difference method, we can enrich for subclasses of neuropeptide-Y-positive GABAergic interneurons by effectively subtracting the expression pattern of one promoter from that of another. These methods harness the complexity of gene expression patterns in the brain and significantly expand the number of genetically tractable neuron classes across mammals. : Mehta et al. describe methods for accessing inhibitory neuron subclasses in the mammalian forebrain. Keywords: inhibitory neurons, somatostatin, parvalbumin, neuropeptide-Y, cell type-specific promoter, adeno-associated virus, rodent, marmoset, macaque, functional in vivo imaging

Published in Cell Reports

ISSN: 2211-1247 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: http://www.cell.com/cell-reports/home

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