Biochemical and molecular study of glycinebetaine synthesis in Salsola aucheri under salt stress

Abstract

Salinity promotes the generation of reactive oxygen species (ROS) and oxidative damages of different cellular components. Thus, salt stress tolerance requires activation of antioxidative pathways to protect plant cells from damages. In this study, seedlings of Salsola aucheri were treated with 100, 200, 300, 400 and 500 mM of NaCl and consequently, the activities of some antioxidant enzymes were evaluated. Enhanced activities of catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) were determined by the increasing salinity up to 300 mM. Furthermore, accumulation of glycinebetaine (GB) as a common response to salt stress was assessed. Accordingly, the concentration of GB enhanced by the increasing concentration of NaCl up to 300 mM and decreased at NaCl concentrations of 400 and 500 mM. Likewise, assessing the activity of betaine aldehyde dehydrogenase (BADH) as a key enzyme in the biosynthesis of GB showed that BADH activity enhanced by the increasing salinity (up to 300 mM NaCl) and decreased at the higher concentrations of NaCl. Cloning and characterization of BADH cDNA from Salsola aucheri and the expression pattern of BADH transcript were also examined. An open reading frame of 797 bp encoded a protein which showed high homology to BADH enzymes in other plants. These results suggest that the salinity tolerance of Salsola aucheri might be closely associated with the increasing function of the antioxidative system to scavenge reactive oxygen species and with the accumulation of the osmoprotectant glycinebetaine.

Keywords

• Salsola aucheri
• glycinebetaine
• betaine aldehyde dehydrogenase
• salinity