Process development for Trastuzumab production from Chinese Hamster Ovary (CHO) in laboratory scale

Abstract

Background: CHO cells have been commonly used in the production of recombinant protein. It is reported that around 70% recombinant therapeutic proteins are produced in CHO cells systems and the market size exceed 15£ billion per year. With the optimization of culture conditions showed that CHO cell density can reach 18 million per cells per milliliter and final product concentration of 01 - 05 g l-1. Therefore, the production process development in laboratory scale plays an important role in identifying the optimal conditions suitable, which can be applied to produce Trastuzumab in industrial scale. In this study, Trastuzumab-producing cell lines were investigated in step wise: culture medium, percentage of supplements, culture temperature and shaking speed. The result showed that the optimal conditions as follows: cells were shaken at 100rpm in ActiPro medium supplemented with Cell Boost 7a/7b (ratio 1:0.1%) daily from day 03, the initial growth temperature was 37oC then shifted to 32oC on day 05. After 14 days, cell density reaches over 25 million per cells per milliliter and Trastuzumab concentration collects around 04 g/L. ntration collects around 04 g/L.

Keywords

• cho
• môi trường nuôi cấy
• trastuzumab