Genome-Scale Mining of Novel Anchor Proteins of Corynebacterium glutamicum

Kerui Lin; Kerui Lin; Nannan Zhao; Nannan Zhao; Youhua Cai; Ying Lin; Ying Lin; Shuangyan Han; Shuangyan Han; Suiping Zheng; Suiping Zheng

doi:10.3389/fmicb.2021.677702

Frontiers in Microbiology (Feb 2022)

Genome-Scale Mining of Novel Anchor Proteins of Corynebacterium glutamicum

Kerui Lin,
Kerui Lin,
Nannan Zhao,
Nannan Zhao,
Youhua Cai,
Ying Lin,
Ying Lin,
Shuangyan Han,
Shuangyan Han,
Suiping Zheng,
Suiping Zheng

Affiliations

Kerui Lin: Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China
Kerui Lin: Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China
Nannan Zhao: Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China
Nannan Zhao: Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China
Youhua Cai: Star Lake Bioscience Co. Inc., Zhaoqing Guangdong, Zhaoqing, China
Ying Lin: Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China
Ying Lin: Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China
Shuangyan Han: Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China
Shuangyan Han: Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China
Suiping Zheng: Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China
Suiping Zheng: Guangdong Research Center of Industrial Enzyme and Green Manufacturing Technology, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China

DOI: https://doi.org/10.3389/fmicb.2021.677702
Journal volume & issue: Vol. 12

Abstract

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The display of recombinant proteins on the surfaces of bacteria is a research topic with many possible biotechnology applications—among which, the choice of host cell and anchoring motif is the key for efficient display. Corynebacterium glutamicum is a promising host for surface display due to its natural advantages, while single screening methods and fewer anchor proteins restrict its application. In this study, the subcellular localization (SCL) predictor LocateP and tied-mixture hidden Markov models were used to analyze all five known endogenous anchor proteins of C. glutamicum and test the accuracy of the predictions. Using these two tools, the SCLs of all proteins encoded by the genome of C. glutamicum 13032 were predicted, and 14 potential anchor proteins were screened. Compared with the positive controls NCgl1221 and NCgl1337, three anchoring proteins—NCgl1307, NCgl2775, and NCgl0717—performed better. This study also discussed the applicability of the anchor protein screening method used in this experiment to other bacteria.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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