Establishment of a non-integrate iPS cell line CSUASOi002-A, from urine-derived cells of a female patient with macular corneal dystrophy carrying compound heterozygous CHST6 mutations

Yutong Jing; Yalan Zhou; Congxiang Wang; Jian Liu; Yonglong Guo; Shengru Mao; Hon fai Chan; Shibo Tang; Jiansu Chen

Stem Cell Research (Dec 2019)

Establishment of a non-integrate iPS cell line CSUASOi002-A, from urine-derived cells of a female patient with macular corneal dystrophy carrying compound heterozygous CHST6 mutations

Yutong Jing,
Yalan Zhou,
Congxiang Wang,
Jian Liu,
Yonglong Guo,
Shengru Mao,
Hon fai Chan,
Shibo Tang,
Jiansu Chen

Affiliations

Yutong Jing: Aier School of Ophthalmology, Central South University, P.R.C., 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China; Aier Eye Institute, 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China
Yalan Zhou: Aier School of Ophthalmology, Central South University, P.R.C., 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China; Aier Eye Institute, 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China
Congxiang Wang: Changsha Aier Eye Hospital, #388 Furong Middle Road, Changsha, Hunan 410015, RP China
Jian Liu: Aier Eye Institute, 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China
Yonglong Guo: Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, RP China
Shengru Mao: Aier School of Ophthalmology, Central South University, P.R.C., 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China; Aier Eye Institute, 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China
Hon fai Chan: Institute for Tissue Engineering and Regenerative Medicine, The Chinese University of Hong Kong, Hong Kong, PR China; School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, PR China
Shibo Tang: Aier School of Ophthalmology, Central South University, P.R.C., 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China; Aier Eye Institute, 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China; CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai 200031, PR China; Corresponding author.
Jiansu Chen: Aier School of Ophthalmology, Central South University, P.R.C., 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China; Aier Eye Institute, 18th floor, the New century building, #198 Furong Middle Road, Changsha, Hunan 410015, PR China; Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, RP China; Institute of Ophthalmology, Medical College, Jinan University, Guangzhou 510632, RP China; Corresponding author.

Journal volume & issue: Vol. 41

Abstract

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We report the human induced pluripotent stem cell line (iPSC) CSUASOi002-A, generated from urine-derived cells (UCs) from a 51-year-old female patient carrying compound heterozygous mutations (c.62_63delTinsGA and c.C892T) in the carbohydrate sulfotransferase 6 gene (CHST6). This patient was from a Chinese family of three siblings with macular corneal dystrophy (MCD). Patient UCs were reprogrammed by electroporation using the episomal plasmids (OCT4, SOX2, KLF4, l-MYC, LIN28 and shP53). The human MCD-UiPS cell line CSUASOi002-A retained the disease-associated genotype, while expressed pluripotent stem cell markers and could be differentiated into cells of all three germ layers.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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