Nature Communications (Aug 2019)

Solid-state NMR spectroscopy based atomistic view of a membrane protein unfolding pathway

  • Peng Xiao,
  • David Bolton,
  • Rachel A. Munro,
  • Leonid S. Brown,
  • Vladimir Ladizhansky

DOI
https://doi.org/10.1038/s41467-019-11849-8
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 11

Abstract

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Studying the unfolding of membrane proteins in a native-like lipid environment is challenging. Here the authors describe a method combining hydrogen-deuterium exchange and solid-state NMR measurements that allows the characterization of unfolding events in lipid-embedded membrane proteins and use the photoreceptor Anabaena Sensory Rhodopsin as a test case.