Cu(II)-baicalein enhance paracrine effect and regenerative function of stem cells in patients with diabetes

Kaijing Liu; Ruihao Li; Shusen Wang; Xue Fu; Ni Zhu; Xiaoyu Liang; Huiyang Li; Xiaoli Wang; Le Wang; Yongjun Li; Jianwu Dai; Jing Yang

Bioactive Materials (Jun 2024)

Cu(II)-baicalein enhance paracrine effect and regenerative function of stem cells in patients with diabetes

Kaijing Liu,
Ruihao Li,
Shusen Wang,
Xue Fu,
Ni Zhu,
Xiaoyu Liang,
Huiyang Li,
Xiaoli Wang,
Le Wang,
Yongjun Li,
Jianwu Dai,
Jing Yang

Affiliations

Kaijing Liu: Key Laboratory of Advanced Medical Materials and Devices, Institute of Biomedical Engineering, Tianjin Institutes of Health Science, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin, 300192, China
Ruihao Li: Department of Vascular Surgery, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, 100730, China; Graduate School of Peking Union Medical College, Beijing, 100730, China
Shusen Wang: Organ Transplant Center, Tianjin First Central Hospital, Nankai University, Tianjin, China; Tianjin Clinical Research Center for Organ Transplantation, Tianjin, China
Xue Fu: Key Laboratory of Advanced Medical Materials and Devices, Institute of Biomedical Engineering, Tianjin Institutes of Health Science, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin, 300192, China
Ni Zhu: Key Laboratory of Advanced Medical Materials and Devices, Institute of Biomedical Engineering, Tianjin Institutes of Health Science, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin, 300192, China
Xiaoyu Liang: Key Laboratory of Advanced Medical Materials and Devices, Institute of Biomedical Engineering, Tianjin Institutes of Health Science, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin, 300192, China
Huiyang Li: Key Laboratory of Advanced Medical Materials and Devices, Institute of Biomedical Engineering, Tianjin Institutes of Health Science, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin, 300192, China
Xiaoli Wang: Key Laboratory of Advanced Medical Materials and Devices, Institute of Biomedical Engineering, Tianjin Institutes of Health Science, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin, 300192, China; Corresponding author.
Le Wang: Organ Transplant Center, Tianjin First Central Hospital, Nankai University, Tianjin, China; Tianjin Clinical Research Center for Organ Transplantation, Tianjin, China; Corresponding author. Organ Transplant Center, Tianjin First Central Hospital, Nankai University, Tianjin, China.
Yongjun Li: Department of Vascular Surgery, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, 100730, China; Graduate School of Peking Union Medical College, Beijing, 100730, China; Corresponding author. Department of Vascular Surgery, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, 100730, China.
Jianwu Dai: Key Laboratory of Advanced Medical Materials and Devices, Institute of Biomedical Engineering, Tianjin Institutes of Health Science, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin, 300192, China
Jing Yang: Key Laboratory of Advanced Medical Materials and Devices, Institute of Biomedical Engineering, Tianjin Institutes of Health Science, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin, 300192, China; Tianjin Medical Health Research Institute, Tianjin, 300192, China; Corresponding author. Key Laboratory of Advanced Medical Materials and Devices, Institute of Biomedical Engineering, Tianjin Institutes of Health Science, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin, 300192, China.

Journal volume & issue: Vol. 36
pp. 455 – 473

Abstract

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The development of engineered or modified autologous stem cells is an effective strategy to improve the efficacy of stem cell therapy. In this study, the stemness and functionality of adipose stem cells derived from type 1 diabetic donors (T1DM-ASC) were enhanced by treatment with Cu(II)-baicalein microflowers (Cu-MON). After treatment with Cu-MON, T1DM-ASC showed enhanced expression of the genes involved in the cytokine-cytokine receptor interaction pathway and increased cytokine secretion. Among the top 13 differentially expressed genes between T1DM-ASC and Cu-MON-treated T1DM-ASC (CMTA), some genes were also expressed in HUVEC, Myoblast, Myofibroblast, and Vascular Smooth Muscle cells, inferring the common role of these cell types. In vivo experiments showed that CMTA had the same therapeutic effect as adipose-derived stem cells from non-diabetic donors (ND-ASC) at a 15% cell dose, greatly reducing the treatment cost. Taken together, these findings suggest that Cu-MON promoted angiogenesis by promoting the stemness and functionality of T1DM-ASC and influencing multiple overall repair processes, including paracrine effects.

Published in Bioactive Materials

ISSN: 2452-199X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials; Science: Biology (General)
Website: https://www.keaipublishing.com/en/journals/bioactive-materials/

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