Frontiers in Bioengineering and Biotechnology (Sep 2022)

A sensitive and rapid method of lead detection using nanoparticle technology based on monoclonal antibody

  • Kunzhi Jia,
  • Ming Lin,
  • Qiang Zhao,
  • Mingke Dong,
  • Sumei Ling,
  • Shihua Wang

DOI
https://doi.org/10.3389/fbioe.2022.962230
Journal volume & issue
Vol. 10

Abstract

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Lead (Pb) threatens public health due to its toxicity and nonbiodegradable characteristics. It is of significance to develop a sensitive and rapid method for Pb detection. In this study, monoclonal antibodies against Pb were screened with a high affinity constant (Kaff) of 3.56 × 109 L/mol. Au nanosphere particles (AuNS) and Au nanoflower particles (AuNF) were synthesized with a diameter of 15 nm and 60 nm, respectively. The specific anti-Pb antibodies were then immobilized on AuNS and AuNF for probe development. At last, AuNS- and AuNF-based strips were successfully assembled for comparative study, which were able to effectively detect environmental Pb in 10 min. The limits of detection (LODs) were determined to be 3.91 ng/ml and 0.2 ng/ml, respectively. Thus the developed method provides a feasible solution for sensitive and rapid detection of Pb on site, which is beneficial to food safety and pollution control.

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