Cervical cancer treatment of Co(II) coordination polymer through miR-9-5p-regulated BRCA1-OCT1-GADD45 pathways

Abstract

Fresh Co(II) concluding coordination polymer based on {[Co(μ-ppda)(μ-pbmeix)]∙0.5pbmeix·H2O}n (1) was formed smoothly through the reaction of Co(II) salt with p-phenylenediacetic acid (H2ppda) under the conditions of N-donor co-ligand semirigid 1,4-bis(2-methylimidazol-1-ylmethyl)benzene (pbmeix) for cervical cancer therapy. In our biological research, we explored the pathogenesis of cervical cancer and provided new targets for cervical cancer treatment. In patients with cervical cancer and the cervical cancer rat model, the expression of Breast Cancer Susceptibility Protein-1 (BRCA1) was aberrantly upregulated. This phenomenon may play a role in the occurrence and development of cervical cancer. Then, bioinformatics prediction was conducted, and miR-9-5p was speculated as the up-regulator of BRCA1 in cervical cancer cells. The influence of miR-9-5p on the vascular endothelial growth factor and pigment epithelium-derived factor contents of cervical cancer lesions was determined via enzyme‐linked immunosorbent (ELISA) assay. Then, the proliferation of cervical cancer cells was determined via CCK-8 assay after miR-9-5p transfection. Finally, we proved that complex 1 is an excellent candidate for cervical cancer therapy through miR-9-5p-regulated BRCA1-OCT1-GADD45 pathways.

Keywords

• Coordination polymer
• Cervical cancer
• microRNA
• miR-9-5p
• BRCA1