Effects of Daegangwhal-Tang Hot Aqueous Extract on Anti-inflammation and Anti-oxidation in RAW 264.7 Macrophage

Abstract

Background The objective of this study was to determine the effects of Daegangwhal-Tang (DGHT) hot aqueous extract on production of inflammatory mediators and antioxidants in RAW 264.7 macrophage. Methods DGHT was extracted with water, filtered, concentrated and freeze-dried to perform. Cytotoxicity of DGHT extract was performed by MTT assay. Activated macrophages were treated with varying concentrations of DGHT extract (10, 50, 100 and 200 μg/mL), and nitric oxide (NO) and prostaglandin E2(PGE2) concentrations were measured to detect anti-oxidative effects. Interleukin-6 (IL-6), interleukin-1 beta (IL-1ß) and tumor necrosis factor-alpha(TNF-α) concentrations were also measured to detect inflammatory responses to DGHT Results Cytotoxicity of DGHT extract at concentrations of 10, 50, 100 and 200 μg/mL were not observed. NO production was significantly decreased in the DGHT hot aqueous extract 200 μg/mL concentration group. PGE2, IL-6, IL-1ß and TNF-α production was significantly decreased in the DGHT hot aqueous extract 100 and 200 μg/mL concentration groups. DGHT hot aqueous extract appeared to have DPPH free radical scavenging capability at all of concentrations, but did not exceed 50%. Conclusions These results suggest that DGHT hot aqueous extract has concentration-dependent anti-inflammatory and anti-oxidative effect.

Keywords

• inflammation
• antioxidant
• herbal medicine
• macrophage