Target preference of Type III-A CRISPR-Cas complexes at the transcription bubble

Tina Y. Liu; Jun-Jie Liu; Abhishek J. Aditham; Eva Nogales; Jennifer A. Doudna

doi:10.1038/s41467-019-10780-2

Nature Communications (Jul 2019)

Target preference of Type III-A CRISPR-Cas complexes at the transcription bubble

Tina Y. Liu,
Jun-Jie Liu,
Abhishek J. Aditham,
Eva Nogales,
Jennifer A. Doudna

Affiliations

Tina Y. Liu: Department of Molecular and Cell Biology, University of California
Jun-Jie Liu: Department of Molecular and Cell Biology, University of California
Abhishek J. Aditham: Department of Chemical & Biomolecular Engineering, University of California
Eva Nogales: Department of Molecular and Cell Biology, University of California
Jennifer A. Doudna: Department of Molecular and Cell Biology, University of California

DOI: https://doi.org/10.1038/s41467-019-10780-2
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 13

Abstract

Read online

Type III CRISPR-Cas systems are able to target transcriptionally active DNA sequences in phages and plasmids. Here, the authors reveal the mechanism of the target nucleic acid preference of Type III-A CRISPR-Cas complexes at the transcription bubble by a combination of structural and biochemical approaches.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal