Mitigation of Chilling Stress by Ozone Pretreatment and Acclimation of Sweet Pepper Grown under Unheated Greenhouse Conditions

Abstract

Ozone is an important air pollutant that causes many challenges for human health, such as lung diseases. The negative impacts of exogenous ozone on cultivated plants have been discussed in several publications, but the positive impacts are less investigated. The current study is an attempt to answer the following question: is there any positive contribution of ozone pretreatment in growing plants under stress? Plants grown in unheated plastic greenhouses suffer from cold stress during the winter when the temperature drops to 5–10 °C. This stress can also be enhanced under saline soil conditions in arid regions. Treatments involving different ozone application methods (seed priming and seedling foliar application) and cold pretreatment (4 °C for 36 h) were compared with untreated controls of two sweet pepper hybrids (Zidenka and Lirica) in an unheated plastic greenhouse. In general, the Lirica hybrid outperformed the Zidenka hybrid in growth and fruit yield and showed good adaptation to cold stress resulting from ozone treatment through foliar application on seedlings. Moreover, cold treatment was associated with higher values of the studied parameters compared to the control (untreated). A concentration of 20 ppm of ozone in the first season and as 30 ppm of ozone in the second season increased plant leaf area, proline content, and APX activity, all of which improved the plant’s defense mechanisms against cold stress. These benefits contributed to high fruit sets; early, total, and marketable fruit yields; and fruit quality under cold stress. The highest yield (16.52 kg m−2) was attained with 30 ppm ozone applied as a seedling spray, compared with 10.07 kg m−2 in the control. Therefore, the foliar application of ozone up to 30 ppm can be recommended for sweet pepper hybrids of Lirica under unheated plastic greenhouse conditions. Further investigations are needed to study the interaction of combined ozone and cold stress, as well as various levels of soil salinity.

Keywords

• proline
• ascorbate peroxidase
• membrane permeability
• yield and quality
• cold pretreatment