Fabrication of micro-patterned titanium dioxide nanotubes thin film and its biocompatibility

Abstract

In this study, in order to obtain a novel biomaterials surface which possesses the functions of an anticoagulant, inhibiting smooth muscle proliferation and pro-endothelialisation simultaneously, a micro-patterned titanium dioxide (TiO(2))-nanotubes (P-TiO(2)-nanotubes) thin film was prepared on titanium (Ti) surface by photolithography combining with anodising method. The authors found that the P-TiO(2)-nanotubes could reduce platelet attachment and conformational change of fibrinogen (FGN) as well as promoting endothelial cells (ECs) growth compared with the TiO(2)-nanotubes. Notably, P-TiO(2)-nanotubes could significantly reduce smooth muscle cells (SMCs) proliferation compared with TiO(2)-nanotubes and TiO(2), and the ECs and SMCs cultured on the P-TiO(2)-nanotubes thin film were elongated, which was suggested to be beneficial for maintaining the cell function. Therefore it is suggested that the P-TiO(2)-nanotubes thin film can contribute more to biocompatible functions of regulating and coordinating the behaviour of platelets, ECs and SMCs.

Keywords

• titanium compounds
• nanobiotechnology
• cellular biophysics
• nanofabrication
• nanotubes
• thin films
• micropatterned titanium dioxide nanotubes thin film
• biocompatibility
• biomaterials surface
• anticoagulant
• smooth muscle proliferation
• proendothelialisation
• photolithography
• endothelial cells growth
• cell function
• TiO2