Frontiers in Immunology (Aug 2022)
EZH2 restricts Tcf7 DNA methylation and promotes TFH differentiation during acute viral infection
- Yuan Luo,
- Yuan Luo,
- Dan Li,
- Luoyingzi Xie,
- Shun Lei,
- Xiangyu Chen,
- Cong Wang,
- Dong Yao,
- Lin Li,
- Jingyi Fang,
- Cheng Chen,
- Shijie Yuan,
- Fei Li,
- Xiaorong Xie,
- Yan Zhang,
- Zhirong Li,
- Li Hu,
- Jianfang Tang,
- Lilin Ye,
- Zhengping Wei,
- Zhengping Wei,
- Ran He
Affiliations
- Yuan Luo
- Department of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Yuan Luo
- Institute of Immunology, Third Military Medical University, Chongqing, China
- Dan Li
- Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Luoyingzi Xie
- Institute of Immunology, Third Military Medical University, Chongqing, China
- Shun Lei
- Institute of Immunology, Third Military Medical University, Chongqing, China
- Xiangyu Chen
- Institute of Immunology, Third Military Medical University, Chongqing, China
- Cong Wang
- Chongqing General Hospital, University of Chinese Academy of Sciences, Chongqing, China
- Dong Yao
- Department of Anesthesiology, Chongqing Public Health Medical Center, Chongqing, China
- Lin Li
- Department of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Jingyi Fang
- Institute of Immunology, Third Military Medical University, Chongqing, China
- Cheng Chen
- Institute of Immunology, Third Military Medical University, Chongqing, China
- Shijie Yuan
- Department of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Fei Li
- Department of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Xiaorong Xie
- Department of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Yan Zhang
- Institute of Immunology, Third Military Medical University, Chongqing, China
- Zhirong Li
- Institute of Immunology, Third Military Medical University, Chongqing, China
- Li Hu
- Institute of Immunology, Third Military Medical University, Chongqing, China
- Jianfang Tang
- Institute of Immunology, Third Military Medical University, Chongqing, China
- Lilin Ye
- Institute of Immunology, Third Military Medical University, Chongqing, China
- Zhengping Wei
- Institute of Immunology, Third Military Medical University, Chongqing, China
- Zhengping Wei
- Dermatology Hospital, Southern Medical University, Guangzhou, China
- Ran He
- Department of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- DOI
- https://doi.org/10.3389/fimmu.2022.942465
- Journal volume & issue
-
Vol. 13
Abstract
Follicular helper T (TFH) cells provide specialized help for B cells to ensure optimal humoral immunity. The histone methyltransferase EZH2, as a chromatin repressor, secures the TFH differentiation by promoting TFH lineage associated gene expression during acute viral infection, including Tcf7 and Bcl6. By using conditional deletion murine system, we observed that EZH2 ablation in CD4+ T cells was accompanied by aberrant accumulation of DNA methyltransferases (DNMTs) DNMT1 and DNMT3B in TFH cells. And the loss of EZH2 promoted aggravation of DNA methylation status at Tcf7 locus. Therefore, our findings suggested that EZH2 plays an important role in maintenance of hypomethylation at Tcf7 locus thus affecting TFH differentiation during acute viral infection.
Keywords
