Study about the effectivity of RIZOBAC in the process of in potato callogenesis var. Desiree

Abstract

This experiment was carried out in a plant biotechnology laboratory at Havana Agronomy University (UNAH), evaluating the effects of different concentrations of bacterial preparations (RIZOBAC) on the obtention of fresh mass and on embryogenesis caracteristics of potato (Solanum tuberosum, L.) var. Desirée, callus in vitro obtained from the potato Germoplasm Bank of the Departament of Genetics and Plant Improvement, at the National Institute of Agricultural Science (INCA) as well as to estimate the possible substitution of plant growth regulators such as 2,4-D and kynetine in the culture media, to establish callogenesis of this crop. Different treatments were studied employing the established crop media as the control treatment; besides, the substitution of the auxins (3.0 mg.l-1) and citokinins (0.5 mg.l-1), with the bioactive complex (RIZOBAC) in different concentrations (0.5; 1.0; 1.5 y 2.0 mg.l-1), and in other cases adding to the control culture media, the same concentration. The fresh weight, colour changes, texture and consistence of the callus were evaluated. Variations were noted in the clone response as well as in the indicators. The best results were obteined using (1.5 mg.l-1) RIZOBAC as a sustitute for auxins. Favorable results were also obtained when the (RIZOBAC) at concentration of 1, 5 mg.l-1 was included in the original culture media that contained the two plant growth regulators. More than 10cts USD could be saved per litre of culture media with the substitution of 2, 4-D for the RIZOBAC. Keywords: biopreparates, callus, indices, in vitro, plant growth regulator