Derivation of dorsal spinal sensory interneurons from human pluripotent stem cells

Sandeep Gupta; Ken Yamauchi; Bennett G. Novitch; Samantha J. Butler

STAR Protocols (Mar 2021)

Derivation of dorsal spinal sensory interneurons from human pluripotent stem cells

Sandeep Gupta,
Ken Yamauchi,
Bennett G. Novitch,
Samantha J. Butler

Affiliations

Sandeep Gupta: Department of Neurobiology, University of California Los Angeles, Los Angeles, CA 90095, USA
Ken Yamauchi: Department of Neurobiology, University of California Los Angeles, Los Angeles, CA 90095, USA; Eli & Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California Los Angeles, Los Angeles, CA 90095, USA
Bennett G. Novitch: Department of Neurobiology, University of California Los Angeles, Los Angeles, CA 90095, USA; Eli & Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California Los Angeles, Los Angeles, CA 90095, USA; Intellectual & Developmental Disabilities Research Center, University of California Los Angeles, Los Angeles, CA 90095, USA
Samantha J. Butler: Department of Neurobiology, University of California Los Angeles, Los Angeles, CA 90095, USA; Eli & Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California Los Angeles, Los Angeles, CA 90095, USA; Intellectual & Developmental Disabilities Research Center, University of California Los Angeles, Los Angeles, CA 90095, USA; Corresponding author

Journal volume & issue: Vol. 2, no. 1
p. 100319

Abstract

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Summary: We describe two differentiation protocols to derive sensory spinal interneurons (INs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). In protocol 1, we use retinoic acid (RA) to induce pain, itch, and heat mediating dI4/dI6 interneurons, and in protocol 2, RA with bone morphogenetic protein 4 (RA+BMP4) is used to induce proprioceptive dI1s and mechanosensory dI3s in hPSC cultures. These protocols provide an important step toward developing therapies for regaining sensation in spinal cord injury patients.For complete details on the use and execution of this protocol, please refer to Gupta et al. (2018).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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