Antioxidant and anti-inflammatory activities of Ganoderma resinaceum (Boud) fruiting bodies extracts

Marius Trésor Kemegne Sipping; Francine Kengne Mediesse; Annette Yannuvie Natia Sombes; Adamou Mfopa; Thaddée Boudjeko

doi:10.34172/jhp.2022.40

Journal of HerbMed Pharmacology (Jul 2022)

Antioxidant and anti-inflammatory activities of Ganoderma resinaceum (Boud) fruiting bodies extracts

Marius Trésor Kemegne Sipping,
Francine Kengne Mediesse,
Annette Yannuvie Natia Sombes,
Adamou Mfopa,
Thaddée Boudjeko

Affiliations

Marius Trésor Kemegne Sipping: Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre-Nkolbisson, University of Yaoundé 1, P.O. Box 17673, Etetak, Yaoundé, Cameroon
Francine Kengne Mediesse: Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre-Nkolbisson, University of Yaoundé 1, P.O. Box 17673, Etetak, Yaoundé, Cameroon
Annette Yannuvie Natia Sombes: Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre-Nkolbisson, University of Yaoundé 1, P.O. Box 17673, Etetak, Yaoundé, Cameroon
Adamou Mfopa: Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre-Nkolbisson, University of Yaoundé 1, P.O. Box 17673, Etetak, Yaoundé, Cameroon
Thaddée Boudjeko: Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre-Nkolbisson, University of Yaoundé 1, P.O. Box 17673, Etetak, Yaoundé, Cameroon

DOI: https://doi.org/10.34172/jhp.2022.40
Journal volume & issue: Vol. 11, no. 3
pp. 348 – 359

Abstract

Read online

Introduction: Ganoderma resinaceum is used to treat oxidative and inflammatory-related diseases such as cardiovascular and liver diseases. Thus, this study aimed to evaluate the antioxidant and anti-inflammatory activities of different extracts from G. resinaceum fruiting bodies. Methods: Aqueous crude (GRT), mycelial (MYC), exopolysaccharide (EPS I, EPS II) and water-soluble polysaccharide-rich (GRP I and GRP II) extracts of G. resinaceum were assessed for their free radical scavenging and metal chelating ions assays. The in vitro anti-inflammatory activity was evaluated by stabilization of erythrocytes’ membranes and protein denaturation assays. For the in vivo study, paw oedema was induced by administration of κ-carrageenan (0.1 mL; 1%) to male Wistar rats aged 4 to 6 weeks. Animals were pre-treated with G. resinaceum extracts (125 mg/kg) and diclofenac sodium (20 mg/kg). Inflammatory cytokine and chemokine levels were determined, and histological analysis of paw tissue was performed. Results: G. resinaceum polysaccharide-rich extracts (GRP I and GRP II) showed the best bioactivities. They scavenged DPPH (1,1-diphenyl-2-picrylhydrazyl, ABTS (2,2-azino-bis-3- ethylbenzylthiazoline-6-sulfonic acid, and NO (nitric oxide) radicals, and chelated ferrous ions, stabilized murine erythrocyte membranes, and inhibited protein denaturation. At 125 mg/kg, GRP I and GRP II restored the microarchitecture with a weak infiltration of immune cells in the subcutaneous tissues. Moreover, they decreased the overproduction of proinflammatory cytokines growth colony-stimulating factor (G-CSF), interferon gamma (IFNγ), tumour necrosis factor alpha (TNFα), chemokines (eotaxin, fractalkine) and increased the levels of anti-inflammatory cytokines (IL-10, IL-12p70). Conclusion: G. resinaceum polysaccharide extracts could be potent antioxidant and antiinflammatory agents.

Published in Journal of HerbMed Pharmacology

ISSN: 2345-5004 (Online)
Publisher: Shahrekord University of Medical Sciences
Country of publisher: Iran, Islamic Republic of
LCC subjects: Medicine: Medicine (General); Medicine: Therapeutics. Pharmacology
Website: http://www.herbmedpharmacol.com/

About the journal

Abstract

Keywords