Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells

Ishan Agrawal; Nidhi Sharma; Shivanjali Saxena; S. Arvind; Debayani Chakraborty; Debarati Bhunia Chakraborty; Deepak Jha; Surajit Ghatak; Sridhar Epari; Tejpal Gupta; Sushmita Jha

STAR Protocols (Sep 2021)

Protocol for induction and characterization of microglia extracellular traps in murine and human microglia cells

Ishan Agrawal,
Nidhi Sharma,
Shivanjali Saxena,
S. Arvind,
Debayani Chakraborty,
Debarati Bhunia Chakraborty,
Deepak Jha,
Surajit Ghatak,
Sridhar Epari,
Tejpal Gupta,
Sushmita Jha

Affiliations

Ishan Agrawal: Department of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, India; Corresponding author
Nidhi Sharma: Department of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, India; Karolinska Institute and Scilifelab, Stockholm, Sweden
Shivanjali Saxena: Department of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, India
S. Arvind: Department of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, India
Debayani Chakraborty: Department of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, India
Debarati Bhunia Chakraborty: Department of Computer Science and Engineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, India
Deepak Jha: Department of Neurosurgery, All India Institute of Medical Sciences Jodhpur, Jodhpur, Rajasthan 342005, India
Surajit Ghatak: Department of Anatomy, All India Institute of Medical Sciences Jodhpur, Jodhpur, Rajasthan 342005, India
Sridhar Epari: Department of Pathology, Tata Memorial Hospital, Mumbai, Maharashtra 400012, India
Tejpal Gupta: Department of Radiation Oncology, Tata Memorial Hospital, Mumbai, Maharashtra 400012, India
Sushmita Jha: Department of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, India; Corresponding author

Journal volume & issue: Vol. 2, no. 3
p. 100678

Abstract

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Summary: Extracellular traps (ETs) are composed of decondensed chromatin and are embedded with various antimicrobial proteins like myeloperoxidase and histones. Recently, we reported that dopamine (DA) induces ETs in BV2 microglia cell line and primary adult human microglia in a manner independent of cell death, reactive oxygen species, and actin polymerization. This protocol details how to characterize DA-induced ETs in BV2 microglia and human microglia. The protocols for characterization of ETs may also be used for other adherent cell lines.For complete details on the use and execution of this protocol, please refer to Agrawal et al. (2021).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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