Application of a Fluorescent Probe for the Online Measurement of PM-Bound Reactive Oxygen Species in Chamber and Ambient Studies

Reece Brown; Svetlana Stevanovic; Zachary Brown; Mingfu Cai; Shengzhen Zhou; Wei Song; Xinming Wang; Branka Miljevic; Jun Zhao; Steven Bottle; Zoran Ristovski

doi:10.3390/s19204564

Sensors (Oct 2019)

Application of a Fluorescent Probe for the Online Measurement of PM-Bound Reactive Oxygen Species in Chamber and Ambient Studies

Reece Brown,
Svetlana Stevanovic,
Zachary Brown,
Mingfu Cai,
Shengzhen Zhou,
Wei Song,
Xinming Wang,
Branka Miljevic,
Jun Zhao,
Steven Bottle,
Zoran Ristovski

Affiliations

Reece Brown: ILAQH (International Laboratory of Air Quality and Health), Queensland University of Technology (QUT), George St. 2, Brisbane 4000 QLD, Australia
Svetlana Stevanovic: ILAQH (International Laboratory of Air Quality and Health), Queensland University of Technology (QUT), George St. 2, Brisbane 4000 QLD, Australia
Zachary Brown: ILAQH (International Laboratory of Air Quality and Health), Queensland University of Technology (QUT), George St. 2, Brisbane 4000 QLD, Australia
Mingfu Cai: School of Atmospheric Sciences, and Guangdong Province Key Laboratory for Climate Change and Natural Disaster Studies, Sun Yat-sen University, Guangzhou 510275, China
Shengzhen Zhou: School of Atmospheric Sciences, and Guangdong Province Key Laboratory for Climate Change and Natural Disaster Studies, Sun Yat-sen University, Guangzhou 510275, China
Wei Song: State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry (GIG), Chinese Academy of Sciences, Guangzhou 510640, China
Xinming Wang: State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry (GIG), Chinese Academy of Sciences, Guangzhou 510640, China
Branka Miljevic: ILAQH (International Laboratory of Air Quality and Health), Queensland University of Technology (QUT), George St. 2, Brisbane 4000 QLD, Australia
Jun Zhao: School of Atmospheric Sciences, and Guangdong Province Key Laboratory for Climate Change and Natural Disaster Studies, Sun Yat-sen University, Guangzhou 510275, China
Steven Bottle: School of Chemistry, Physics and Mechanical Engineering, Queensland University of Technology (QUT), Brisbane, 4000 QLD, Australia
Zoran Ristovski: ILAQH (International Laboratory of Air Quality and Health), Queensland University of Technology (QUT), George St. 2, Brisbane 4000 QLD, Australia

DOI: https://doi.org/10.3390/s19204564
Journal volume & issue: Vol. 19, no. 20
p. 4564

Abstract

Read online

This manuscript details the application of a profluorescent nitroxide (PFN) for the online quantification of radical concentrations on particulate matter (PM) using an improved Particle Into Nitroxide Quencher (PINQ). A miniature flow-through fluorimeter developed specifically for use with the 9,10-bis(phenylethynyl)anthracene-nitroxide (BPEAnit) probe was integrated into the PINQ, along with automated gas phase corrections through periodic high efficiency particle arrestor (HEPA) filtering. The resulting instrument is capable of unattended sampling and was operated with a minimum time resolution of 2.5 min. Details of the fluorimeter design and examples of data processing are provided, and results from a chamber study of side-stream cigarette smoke and ambient monitoring campaign in Guangzhou, China are presented. Primary cigarette smoke was shown to have both short-lived (t1/2 = 27 min) and long-lived (t1/2 = indefinite) PM-bound reactive oxygen species (ROS) components which had previously only been observed in secondary organic aerosol (SOA).

Published in Sensors

ISSN: 1424-8220 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology
Website: http://www.mdpi.com/journal/sensors

About the journal

Abstract

Keywords