Biotechnology & Biotechnological Equipment (Nov 2017)

Fungal chitosan production using xylose rich of corn stover prehydrolysate by Rhizopus oryzae

  • Lei Yang,
  • Xin Li,
  • Chenhuan Lai,
  • Yimin Fan,
  • Jia Ouyang,
  • Qiang Yong

DOI
https://doi.org/10.1080/13102818.2017.1370678
Journal volume & issue
Vol. 31, no. 6
pp. 1160 – 1166

Abstract

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Xylose rich of lignocellulosic prehydrolysate could be used for fungal chitosan production effectively by Rhizopus oryzae. When 15 g/L xylose was used as carbon source for R. oryzae AS 3.819 fermentation, almost all of the xylose was consumed for cell growth (4.33 g/L of biomass) and the chitosan extraction yield was 0.11 g/g biomass. Corn stover prehydrolysate by dilute acid-assisted steam explosion pretreatment that contained 30 g/L xylose and 10 g/L glucose was fermented by R. oryzae AS 3.819 for 72 h, the biomass and the chitosan extraction yield were 10.96 g/L and 0.09 g/g biomass. The degree of deacetylation of the fungal chitosan derived from corn stover prehydrolysate (91.27%) was higher than that of the commercial chitosan extracted from natural shellfish exoskeleton (87.25%), and the viscosity of the fungal chitosan derived from corn stover prehydrolysate (2.67 mPa⋅s) had a large decrease compared to the commercial chitosan (22.25 mPa⋅s). The functional groups and thermostability of the fungal chitosan were proofed by Infrared (IR) spectra and Derivative thermogravimetric (DTG), respectively.

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