Coenzyme Coupling Boosts Charge Transport through Single Bioactive Enzyme Junctions

Xiaoyan Zhuang; Aihui Zhang; Siyao Qiu; Chun Tang; Shiqiang Zhao; Hongchun Li; Yonghui Zhang; Yali Wang; Binju Wang; Baishan Fang; Wenjing Hong

iScience (Apr 2020)

Coenzyme Coupling Boosts Charge Transport through Single Bioactive Enzyme Junctions

Xiaoyan Zhuang,
Aihui Zhang,
Siyao Qiu,
Chun Tang,
Shiqiang Zhao,
Hongchun Li,
Yonghui Zhang,
Yali Wang,
Binju Wang,
Baishan Fang,
Wenjing Hong

Affiliations

Xiaoyan Zhuang: State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; The Key Laboratory for Chemical Biology of Fujian Province, Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China
Aihui Zhang: State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; The Key Laboratory for Chemical Biology of Fujian Province, Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China
Siyao Qiu: Science & Technology Innovation Institute, Dongguan University of Technology, Dongguan 523018, China
Chun Tang: State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
Shiqiang Zhao: State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
Hongchun Li: Research Center for Computer-Aided Drug Discovery, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China
Yonghui Zhang: College of Food and Biology Engineering, Jimei University, Xiamen 361005, China
Yali Wang: State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; The Key Laboratory for Chemical Biology of Fujian Province, Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China
Binju Wang: State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
Baishan Fang: State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; The Key Laboratory for Chemical Biology of Fujian Province, Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China; Corresponding author
Wenjing Hong: State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; Corresponding author

Journal volume & issue: Vol. 23, no. 4

Abstract

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Summary: Oxidation of formate to CO2 is catalyzed via the donation of electrons from formate dehydrogenase (FDH) to nicotinamide adenine dinucleotide (NAD+), and thus the charge transport characteristics of FDH become essential but remain unexplored. Here, we investigated the charge transport through single-enzyme junctions of FDH using the scanning tunneling microscope break junction technique (STM-BJ). We found that the coupling of NAD+ with FDH boosts the charge transport by ∼2,100%, and the single-enzyme conductance highly correlates with the enzyme activity. The combined flicker noise analysis demonstrated the switching of the coenzyme-mediated charge transport pathway and supported by the significantly reduced HOMO-LUMO gap from calculations. Site-specific mutagenesis analysis demonstrated that FDH-NAD+ stably combined own higher bioactivity and boosts charge transport, and the coupling has been optimized via the natural selection. Our work provides evidence of hydrogen bond coupling in bioactivity but also bridges the charge transport through single-enzyme junctions and enzyme activities. : Analytical Chemistry; Biotechnology; Molecular Biology Subject Areas: Analytical Chemistry, Biotechnology, Molecular Biology

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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