Statistical optimization of novel acemannan polysaccharides assisted TiO2 nanorods based nanofibers for skin cancer application

Abstract

In our work, we report the fabrication of novel aloe vera acemannan polysaccharides mediated green synthesized titanium dioxide nanorods loaded resveratrol drug incorporated poly vinyl alcohol nanofibers against A431 skin cancer cells. An innovative approach using RSM Box-Behnken design showed an R2 value of 97.23, 90.69 and 90.1 for fiber diameter, porosity and pore size demonstrating a good fitting for the experimental values observed. The composite nanofibers were characterized through SEM, XRD and FT-IR analysis. Cell viability percentage of about 37.2% at 500 µg/ml and a good zone of inhibition around 7.5 ± 0.5 mm and 5.4 ± 0.4 mm for S. aureus and P. aeruginosa respectively was observed for our PVA+TNR+RSV nanofibers. An effective scavenging of reactive oxygen species was confirmed from our DCFH-DA assay through real time fluorescence spectroscopy study. Thus, the acemannan carbohydrate polysaccharides adhered nano-composite fibrous scaffold proves to be a potent transdermal patch for melanoma applications.

Keywords

• Aloe vera
• Acemannan polysaccharides
• Green synthesis
• Titanium nanorods
• Box Behnken design
• Electrospinning