Proficient synthesis of zinc oxide nanoparticles from Tabernaemontana heyneana Wall. via green combustion method: Antioxidant, anti-inflammatory, antidiabetic, anticancer and photocatalytic activities

D.J. Manasa; K.R. Chandrashekar; M.A. Pavan Kumar; D. Suresh; D.J. Madhu Kumar; C.R. Ravikumar; Tanima Bhattacharya; H.C. Ananda Murthy

Results in Chemistry (Jan 2021)

Proficient synthesis of zinc oxide nanoparticles from Tabernaemontana heyneana Wall. via green combustion method: Antioxidant, anti-inflammatory, antidiabetic, anticancer and photocatalytic activities

D.J. Manasa,
K.R. Chandrashekar,
M.A. Pavan Kumar,
D. Suresh,
D.J. Madhu Kumar,
C.R. Ravikumar,
Tanima Bhattacharya,
H.C. Ananda Murthy

Affiliations

D.J. Manasa: Department of Applied Botany, Mangalore University, Mangalagangothri, Mangalore 574199, Karnataka, India; Department of Studies in Botany, Davangere University, Shivagangothri, Davangere 577007, Karnataka, India
K.R. Chandrashekar: Department of Applied Botany, Mangalore University, Mangalagangothri, Mangalore 574199, Karnataka, India; Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore 575 018, Karnataka, India; Corresponding authors at: Department of Applied Botany, Mangalore University, Mangalagangothri, Mangalore 574199, Karnataka, India (K.R. Chandrashekar). Department of Applied Chemistry, School of Applied Natural Science, Adama Science and Technology University, PO Box 1888, Adama, Ethiopia (H.C. Ananda Murthy).
M.A. Pavan Kumar: Department of Chemistry, University College of Science, Tumkur University, Tumakuru 572 103, Karnataka, India; Department of Studies in Biochemistry, Jnana Kaveri PG Centre, ChikkaAluvara, Mangalore University, Karnataka, India; Acharya Institute of Graduate Studies, Soladevanahalli, Bengaluru 560 107, Karnataka, India
D. Suresh: Department of Chemistry, University College of Science, Tumkur University, Tumakuru 572 103, Karnataka, India
D.J. Madhu Kumar: PG Department of Chemistry, Davangere University, Shivagangothri, Davangere 577007, Karnataka, India
C.R. Ravikumar: Research Centre, Department of Science, East West Institute of Technology, Bangalore 560091, India
Tanima Bhattacharya: Hubei Collaborative Innovation Centre for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules and College of Chemistry and Chemical Engineering, Hubei University, Wuhan, Hubei 430062, China
H.C. Ananda Murthy: Department of Applied Chemistry, School of Applied Natural Science, Adama Science and Technology University, PO Box 1888, Adama, Ethiopia; Corresponding authors at: Department of Applied Botany, Mangalore University, Mangalagangothri, Mangalore 574199, Karnataka, India (K.R. Chandrashekar). Department of Applied Chemistry, School of Applied Natural Science, Adama Science and Technology University, PO Box 1888, Adama, Ethiopia (H.C. Ananda Murthy).

Journal volume & issue: Vol. 3
p. 100178

Abstract

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The zinc oxide nanoparticles (T-ZnO NPs) were synthesized successfully by the application of leaf, stem and callus extracts of Tabernaemontana heyneana Wall. via the green combustion method. The structural, bonding and morphological features of T. heyneana mediated ZnO NPs (T-ZnO NPs) were explored using XRD (X-ray powder diffraction), UV–Vis (Ultra-violet visible spectroscopy), FESEM-EDS (Field Emission-SEM and energy-dispersive X-ray spectra), DLS (Dynamic light scattering) and FTIR (Fourier transform infrared) techniques. UV–Vis spectra revealed the presence of a band in the region between 370 and 376 nm approving the presence of T-ZnO NPs. XRD, FESEM-EDS, TEM and DLS analysis confirmed the formation of nanosized, spherical shaped, highly stable and pure crystalline wurtzite T-ZnO NPs. FTIR spectra revealed the presence of most probable phytochemicals from plant extracts involved in the processes of reduction and stabilization of T-ZnO NPs. The T-ZnO NPs could effectively inhibit the activity of DPPH (1, 1-Diphenyl-2-picrylhydrazyl) radical (IC50 value between 467.7 and 752.3 µg/ml) exhibiting potent radical scavenging activity. The T-ZnO NPs exhibited strong anti-inflammatory (membrane stabilization) activity. The antidiabetic potential of T-ZnO NPs was assessed and found to exhibit excellent α-glucosidase (IC 50 at 16.3 µg/ml) and α-amylase (IC 50 at 42.3 µg/ml) activity. The cytotoxic effect of T-ZnO NPs was investigated against A549 cell line (human lung cancer) by MTT assay which showed dose dependent response. IC50 values were found to be in the range of 89.47–185.8 µg/ml demonstrating their higher cytotoxic efficacy. AO/EB staining indicated the presence of early apoptotic cells. The percentage degradation of carcinogenic methylene blue dye was studied and an outstanding catalytic activity of 87–100% was recorded by ZnO-NPs within 160 min. Hence, T-ZnO NPs proved to be better candidates for futuristic biomedical and industrial applications.

Published in Results in Chemistry

ISSN: 2211-7156 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Chemistry
Website: https://www.journals.elsevier.com/results-in-chemistry/

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