A dextran-based warming method for preparing leukocyte-rich plasma and its clinical application for endotoxin assay

Abstract

We devised a method using dextran for obtaining leukocyte-rich plasma (LRP) to measure endotoxin in blood. In order to find the optimal temperature for obtaining LRP, the measurement results were examined using samples prepared at 37 and 0°C. Sample separation time of LRP was significantly shorter at 37°C than at 0°C. Endotoxin measurement values showed a strong correlation between the two groups but many of the LRPs made at 37°C had measurements above those of the LRPs prepared at 0°C. The diagnostic accuracy for gram-negative bacterial infection was superior for LRP prepared at 37°C, with sensitivity and specificity of 96.8 and 100%, respectively.

Keywords

• dextran
• endotoxin
• endotoxin-specific limulus amebocyte lysate assay
• leukocyte-rich plasma
• platelet-rich plasma
• polymyxin B