Quantification of caffeoylquinic acids and triterpenes as targeted bioactive compounds of Centella Asiatica in extracts and formulations by liquid chromatography mass spectrometry

Liping Yang; Luke Marney; Armando Alcazar Magana; Jaewoo Choi; Kirsten Wright; Janis Mcferrin; Nora E. Gray; Amala Soumyanath; Jan F. Stevens; Claudia S. Maier

Journal of Chromatography Open (Nov 2023)

Quantification of caffeoylquinic acids and triterpenes as targeted bioactive compounds of Centella Asiatica in extracts and formulations by liquid chromatography mass spectrometry

Liping Yang,
Luke Marney,
Armando Alcazar Magana,
Jaewoo Choi,
Kirsten Wright,
Janis Mcferrin,
Nora E. Gray,
Amala Soumyanath,
Jan F. Stevens,
Claudia S. Maier

Affiliations

Liping Yang: Department of Chemistry, Oregon State University, Corvallis, OR, USA; BENFRA Botanical Dietary Supplements Research Center, Oregon Health and Science University, Portland, OR, USA
Luke Marney: Department of Chemistry, Oregon State University, Corvallis, OR, USA; BENFRA Botanical Dietary Supplements Research Center, Oregon Health and Science University, Portland, OR, USA
Armando Alcazar Magana: Department of Chemistry, Oregon State University, Corvallis, OR, USA; Linus Pauling Institute, Oregon State University, Corvallis, OR, USA; Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada
Jaewoo Choi: BENFRA Botanical Dietary Supplements Research Center, Oregon Health and Science University, Portland, OR, USA; Linus Pauling Institute, Oregon State University, Corvallis, OR, USA
Kirsten Wright: BENFRA Botanical Dietary Supplements Research Center, Oregon Health and Science University, Portland, OR, USA; Department of Neurology, Oregon Health and Science University, Portland, OR, USA
Janis Mcferrin: Oregon's Wild Harvest, Redmond, OR, USA
Nora E. Gray: BENFRA Botanical Dietary Supplements Research Center, Oregon Health and Science University, Portland, OR, USA; Department of Neurology, Oregon Health and Science University, Portland, OR, USA
Amala Soumyanath: BENFRA Botanical Dietary Supplements Research Center, Oregon Health and Science University, Portland, OR, USA; Department of Neurology, Oregon Health and Science University, Portland, OR, USA
Jan F. Stevens: BENFRA Botanical Dietary Supplements Research Center, Oregon Health and Science University, Portland, OR, USA; Linus Pauling Institute, Oregon State University, Corvallis, OR, USA; Department of Pharmaceutical Sciences, Oregon State University, Corvallis, OR, USA
Claudia S. Maier: Department of Chemistry, Oregon State University, Corvallis, OR, USA; BENFRA Botanical Dietary Supplements Research Center, Oregon Health and Science University, Portland, OR, USA; Linus Pauling Institute, Oregon State University, Corvallis, OR, USA; Corresponding author at: Department of Chemistry, Oregon State University, Corvallis, OR, USA.

Journal volume & issue: Vol. 4
p. 100091

Abstract

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Centella asiatica (CA) is a culinary vegetable and well-known functional food that is widely used as a medicinal herb and dietary supplement. CA is rich in pentacyclic triterpenes (TTs), including asiaticoside (AS), madecassoside (MS) and the related aglycones asiatic acid (AA), madecassic acid (MA). Traditionally, TTs have been associated with the bioactivity and health promoting effect of CA. Recently, mono-caffeoylquinic acids (MonoCQAs) and di-caffeoylquinic acids (DiCQAs) have been found to contribute to the bioactivity of CA as well. This work reports an analytical strategy based on liquid chromatography coupled to multiple reaction monitoring mass spectrometry (LC-MRM–MS) for the simultaneous rapid and accurate quantification of 12 bioactive compounds in CA, namely AS, MS, AA, MA, 5-CQA, 4-CQA, 3-CQA, 1,3-DiCQA, 3,4-DiCQA, 1,5-DiCQA, 3,5-DiCQA, 4,5-DiCQA. Method selectivity, accuracy, precision, repeatability, robustness, linearity range, limit of detection (LOD), and limit of quantitation (LOQ) were validated. The validated LC-MRM-MS method has been successfully applied to quantify the 12 bioactive compounds in CA aqueous extracts and two related formulations: a standardized CA product (CAP) used in a phase I clinical trial and formulated CA rodent diets used in preclinical studies. The validated method allows us to support the standardization of CA products used for clinical trials and conduct routine LC-MRM-MS analyses of formulated preclinical diets to confirm correct levels of CA phytochemical markers.

Published in Journal of Chromatography Open

ISSN: 2772-3917 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Chemistry: Analytical chemistry
Website: https://www.journals.elsevier.com/journal-of-chromatography-open

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