Adipose tissue-derived stromal cells acquire endothelial-like features upon reprogramming with SOX18

R.D. Fontijn; J. Favre; B.A. Naaijkens; E. Meinster; N.J. Paauw; S.L. Ragghoe; T.D. Nauta; M.A. van den Broek; E.M. Weijers; H.W. Niessen; P. Koolwijk; A.J. Horrevoets

doi:10.1016/j.scr.2014.09.004

Stem Cell Research (Nov 2014)

Adipose tissue-derived stromal cells acquire endothelial-like features upon reprogramming with SOX18

R.D. Fontijn,
J. Favre,
B.A. Naaijkens,
E. Meinster,
N.J. Paauw,
S.L. Ragghoe,
T.D. Nauta,
M.A. van den Broek,
E.M. Weijers,
H.W. Niessen,
P. Koolwijk,
A.J. Horrevoets

Affiliations

R.D. Fontijn: Department of Molecular Cell Biology and Immunology, VU University Medical Center, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
J. Favre: Department of Molecular Cell Biology and Immunology, VU University Medical Center, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
B.A. Naaijkens: Department of Pathology and Cardiac Surgery, VU University Medical Center, de Boelelaan 117, 1081 HV Amsterdam, The Netherlands
E. Meinster: Department of Pathology and Cardiac Surgery, VU University Medical Center, de Boelelaan 117, 1081 HV Amsterdam, The Netherlands
N.J. Paauw: Department of Molecular Cell Biology and Immunology, VU University Medical Center, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
S.L. Ragghoe: Department of Molecular Cell Biology and Immunology, VU University Medical Center, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
T.D. Nauta: Laboratory for Physiology, Institute for Cardiovascular Research, VU University Medical Center, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
M.A. van den Broek: Laboratory for Physiology, Institute for Cardiovascular Research, VU University Medical Center, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
E.M. Weijers: Laboratory for Physiology, Institute for Cardiovascular Research, VU University Medical Center, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
H.W. Niessen: Department of Pathology and Cardiac Surgery, VU University Medical Center, de Boelelaan 117, 1081 HV Amsterdam, The Netherlands
P. Koolwijk: Laboratory for Physiology, Institute for Cardiovascular Research, VU University Medical Center, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
A.J. Horrevoets: Department of Molecular Cell Biology and Immunology, VU University Medical Center, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands

DOI: https://doi.org/10.1016/j.scr.2014.09.004
Journal volume & issue: Vol. 13, no. 3
pp. 367 – 378

Abstract

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Adipose tissue-derived stromal cells (ASC) form a rich source of autologous cells for use in regenerative medicine. In vitro induction of an endothelial phenotype may improve performance of ASCs in cardiovascular repair. Here, we report on an in vitro strategy using direct reprogramming of ASCs by means of ectopic expression of the endothelial-specific transcription factor SRY (sex determining region Y)-box18 (SOX18). SOX18 induces ASCs to express a set of genes involved in vascular patterning: MMP7, KDR, EFNB2, SEMA3G and CXCR4. Accordingly, SOX18 transduced ASCs reorganize under conditions of shear stress, display VEGF-induced chemotaxis and form tubular structures in 3D matrices in an MMP7-dependent manner. These in vitro findings provide insight into molecular and cellular processes downstream of SOX18 and show that reprogramming using SOX18 is sufficient to induce several endothelial-like features in ASCs.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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