Dissolved organic matter characteristics of deciduous and coniferous forests with variable management: different at the source, aligned in the soil

Abstract

Dissolved organic matter (DOM) is part of the biogeochemical cycles of carbon and nutrients, carries pollutants and drives soil formation. The DOM concentration and properties along the water flow path through forest ecosystems depend on its sampling location and transformation processes. To improve our understanding of the effects of forest management, especially tree species selection and management intensity, on DOM concentrations and properties of samples from different ecosystem fluxes, we studied throughfall, stemflow, litter leachate and mineral soil solution at 26 forest sites in the three regions of the German Biodiversity Exploratories. We covered forest stands with three management categories (coniferous, deciduous age class and unmanaged beech forests). In water samples from these forests, we monitored DOC concentrations over 4 years and characterized the quality of DOM with UV-vis absorption, fluorescence spectroscopy combined with parallel factor analysis (PARAFAC) and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS). Additionally, we performed incubation-based biodegradation assays. Multivariate statistics revealed strong significant effects of ecosystem fluxes and smaller effects of main tree species on DOM quality. Coniferous forests differed from deciduous forests by showing larger DOC concentrations, more lignin- and protein-like molecules, and fewer tannin-like molecules in throughfall, stemflow, and litter leachate. Cluster analysis of FT-ICR-MS data indicated that DOM compositions, which varied in aboveground samples depending on tree species, become aligned in mineral soil. This alignment of DOM composition along the water flow path in mineral soil is likely caused by microbial production and consumption of DOM in combination with its interaction with the solid phase, producing a characteristic pattern of organic compounds in forest mineral soils. We found similarly pronounced effects of ecosystem fluxes on the biodegradability of DOM, but surprisingly no differences between deciduous and coniferous forests. Forest management intensity, mainly determined by biomass extraction, contribution of species, which are not site-adapted, and deadwood mass, did not influence DOC concentrations, DOM composition and properties significantly.