Overwintering temperature affects lipid and fatty acid metabolism in hepatopancreas and ovary of female mud crab Scylla paramamosain

Kaijia Yu; Hanying Xu; Ce Shi; Chunlin Wang; Changkao Mu; Yangfang Ye; Shujian Chen; Ronghua Li; Qingyang Wu

Aquaculture Reports (Mar 2025)

Overwintering temperature affects lipid and fatty acid metabolism in hepatopancreas and ovary of female mud crab Scylla paramamosain

Kaijia Yu,
Hanying Xu,
Ce Shi,
Chunlin Wang,
Changkao Mu,
Yangfang Ye,
Shujian Chen,
Ronghua Li,
Qingyang Wu

Affiliations

Kaijia Yu: Marine Economic Research Center, Donghai Academy, Ningbo University, Ningbo, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo, China; Key Laboratory of Aquacultural Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo, China; Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo, China
Hanying Xu: Marine Economic Research Center, Donghai Academy, Ningbo University, Ningbo, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo, China; Key Laboratory of Aquacultural Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo, China; Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo, China; Department of Aquaculture, Zhejiang Ocean University, Zhoushan 316022, China
Ce Shi: Marine Economic Research Center, Donghai Academy, Ningbo University, Ningbo, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo, China; Key Laboratory of Aquacultural Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo, China; Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo, China; Corresponding author at: Marine Economic Research Center, Donghai Academy, Ningbo University, Ningbo, China.
Chunlin Wang: Marine Economic Research Center, Donghai Academy, Ningbo University, Ningbo, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo, China; Key Laboratory of Aquacultural Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo, China; Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo, China
Changkao Mu: Marine Economic Research Center, Donghai Academy, Ningbo University, Ningbo, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo, China; Key Laboratory of Aquacultural Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo, China; Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo, China
Yangfang Ye: Marine Economic Research Center, Donghai Academy, Ningbo University, Ningbo, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo, China; Key Laboratory of Aquacultural Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo, China; Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo, China
Shujian Chen: Marine Economic Research Center, Donghai Academy, Ningbo University, Ningbo, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo, China; Key Laboratory of Aquacultural Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo, China; Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo, China
Ronghua Li: Marine Economic Research Center, Donghai Academy, Ningbo University, Ningbo, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo, China; Key Laboratory of Aquacultural Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo, China; Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo, China
Qingyang Wu: Marine Economic Research Center, Donghai Academy, Ningbo University, Ningbo, China; Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural, Ningbo, China; Key Laboratory of Aquacultural Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo, China; Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo, China

Journal volume & issue: Vol. 40
p. 102563

Abstract

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The influence of four overwintering temperatures, namely 8 ℃, 12 ℃, 16 ℃, and 20 ℃, on lipid metabolism and fatty acid profile in the hepatopancreas and ovary tissues of female mud crab Scylla paramamosain was investigated during eight weeks. According to the results, the hepatosomatic index (HSI) of the crabs after overwintering at 16 ℃ and 20 ℃ was significantly lower than that pre-overwintering (P 0.05). Moreover, the increased overwintering temperature significantly decreased the triglyceride (TG) content in the hepatopancreas (P 0.05). Hemolymph biochemical indices demonstrated that the level of low-density lipoprotein cholesterol (LDL-C) increased markedly as temperature rose (P 0.05), however, the content of EPA in ovary tissue significantly increased with the temperature rise (P < 0.05). Furthermore, the rise in temperature significantly upregulated the expressions of lipid catabolism genes (hsl, cpt Ⅰ) and lipid transport genes (fabp3, fatp4), while significantly downregulated the expressions of lipid synthesis genes (acc, g6pd) in the hepatopancreas. Meanwhile, the temperature did not affect the expressions of lipid synthesis genes (acc, fas, g6pd) and lipid catabolism genes (hsl, aco, cpt Ⅰ, cpt Ⅱ) in ovary tissue. Still, the temperature increase significantly upregulated the expressions of PUFA synthesis genes (Δ6fad, elovl4). In conclusion, overwintering at 16 ℃ and 20 ℃ promoted the process of lipid catabolism and transport in hepatopancreas tissue of mud crabs. However, overwintering at 20 ℃ was beneficial for accumulating more EPA in ovary tissue from a breeding perspective.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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