Genetics and Molecular Biology (Jan 2005)

Eucalyptus ESTs corresponding to the protoporphyrinogen IX oxidase enzyme related to the synthesis of heme, chlorophyll, and to the action of herbicides

  • Edivaldo Domingues Velini,
  • Maria Lúcia Bueno Trindade,
  • Elza Alves,
  • Ana Catarina Catâneo,
  • Celso Luis Marino,
  • Ivan de Godoy Maia,
  • Edson Seizo Mori,
  • Edson Luiz Furtado,
  • Iraê Amaral Guerrini,
  • Carlos Frederico Wilcken

DOI
https://doi.org/10.1590/S1415-47572005000400009
Journal volume & issue
Vol. 28, no. 3
pp. 548 – 554

Abstract

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This work was aimed at locating Eucalyptus ESTs corresponding to the PROTOX or PPO enzyme (Protoporphyrinogen IX oxidase, E.C. 1.3.3.4) directly related to resistance to herbicides that promote oxidative stress, changing the functionality of this enzyme. PROTOX, which is the site of action of diphenyl-ether (oxyfluorfen, lactofen, fomesafen), oxadiazole (oxadiazon and oxadiargyl), and aryl triazolinone (sulfentrazone and carfentrazone) herbicides, acts on the synthesis route of porphyrins which is associated with the production of chlorophyll a, catalases, and peroxidases. One cluster and one single read were located, with e-values better than e-70, associated to PROTOX. The alignment results between amino acid sequences indicated that this enzyme is adequately represented in the ESTs database of the FORESTs project.

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