Transition metal ion FRET uncovers K+ regulation of a neurotransmitter/sodium symporter

Christian B. Billesbølle; Jonas S. Mortensen; Azmat Sohail; Solveig G. Schmidt; Lei Shi; Harald H. Sitte; Ulrik Gether; Claus J. Loland

doi:10.1038/ncomms12755

Nature Communications (Sep 2016)

Transition metal ion FRET uncovers K+ regulation of a neurotransmitter/sodium symporter

Christian B. Billesbølle,
Jonas S. Mortensen,
Azmat Sohail,
Solveig G. Schmidt,
Lei Shi,
Harald H. Sitte,
Ulrik Gether,
Claus J. Loland

Affiliations

Christian B. Billesbølle: Department of Neuroscience and Pharmacology, Molecular Neuropharmacology and Genetics Laboratory, Faculty of Health and Medical Sciences, University of Copenhagen
Jonas S. Mortensen: Department of Neuroscience and Pharmacology, Molecular Neuropharmacology and Genetics Laboratory, Faculty of Health and Medical Sciences, University of Copenhagen
Azmat Sohail: Center for Physiology and Pharmacology, Institute of Pharmacology, Medical University Vienna
Solveig G. Schmidt: Department of Neuroscience and Pharmacology, Molecular Neuropharmacology and Genetics Laboratory, Faculty of Health and Medical Sciences, University of Copenhagen
Lei Shi: Computational Chemistry and Molecular Biophysics Unit, National Institute on Drug Abuse, NIH
Harald H. Sitte: Center for Physiology and Pharmacology, Institute of Pharmacology, Medical University Vienna
Ulrik Gether: Department of Neuroscience and Pharmacology, Molecular Neuropharmacology and Genetics Laboratory, Faculty of Health and Medical Sciences, University of Copenhagen
Claus J. Loland: Department of Neuroscience and Pharmacology, Molecular Neuropharmacology and Genetics Laboratory, Faculty of Health and Medical Sciences, University of Copenhagen

DOI: https://doi.org/10.1038/ncomms12755
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 12

Abstract

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The neurotransmitter transporter SERT counter transport K+ to transport serotonin. Here the authors show that the activity of the prokaryotic orthologue LeuT is also modulated by this cation, suggesting a general regulatory role for K+on neutrotrasmitter:sodium symporters function.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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