Новые технологии (Feb 2023)
Adaptation of the method for identification and quantification of sinigrin in mustard seeds
Abstract
The research is devoted to the study on adaptation, verification and implementation of the method for determining sinigrin in mustard seeds and products of its processing, as well as in other types of food raw materials of plant origin. The relevance of the research is determined, on the one hand, by the need to control sinigrin in the technological processes of mustard processing, and, on the other hand, by the lack of effective standardized methods for determining sinigrin in the Russian Federation. The aim of the research is to modify and (or) adapt the most effective methods for the identification and quantification of sinigrin, presented in the publications of foreign researchers. The objectives of the study included construction of calibration graphs; modification of the sample preparation technique to reduce the complexity and duration of the analysis while maintaining the required parameters of accuracy and reproducibility; adaptation of the analysis method using high performance liquid chromatography; verification and approbation of the proposed method using real production samples, i.e. mustard seeds.High performance liquid chromatography has been used as the basic method.As a result of the research, the sample preparation technique has been modified. It has made it possible to reduce labor intensity and reduce the time for its implementation while maintaining the required metrological characteristics. The calibration dependence has been determined and a method for the identification and quantitative analysis of sinigrin in raw materials of plant origin using a high-performance liquid chromatograph equipped with a diode array and mass spectrometric detectors proposed. As a result of verification, the metrological characteristics of the proposed method have been determined: the measurement range is from 0.0005 to 10%, the accuracy index is ± 0.01%. The method has been tested on real objects, mustard seeds, which are the main sources of sinigrin. The proposed method for the identification and quantification of sinigrin can be recommended for use in research and production laboratories equipped with the necessary measuring instruments and testing equipment.
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