Iranian Journal of Parasitology (Jun 2011)

Disseminated Leishmaniasis Caused by Leishmania Tropica in a Puppy from Karaj, Central Iran

  • M Mohebali,
  • A Malmasi,
  • H Hajjaran,
  • S Jamshidi,
  • B Akhoundi,
  • M Rezaei,
  • S Jani­tabar,
  • H Zarei,
  • S Charehdar

Journal volume & issue
Vol. 6, no. 2
pp. 69 – 73

Abstract

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A 5-month old puppy with muco-cutaneous lesions in the chin, around lips and eyes was exam­ined physically and microscopically for leishmaniasis. Muco-cutaneous lesions containing a large num­ber of amastigotes of Leishmania spp. were observed. Amastigotes were also detected in liver and spleen of the puppy. The animal was positive with Dipstick rK39 kit and high level of anti-Leishmania antibodies was detected by direct agglutination test (DAT). DNA, Using PCR-RFLP technique extracted from cultured Leishmania promastigotes and L. tropica was identified. This is the first report of concurrent mucosal and visceral involvement of L. tropica in a puppy from Iran.

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