Tracing the transitions from pluripotency to germ cell fate with CRISPR screening

Jamie A. Hackett; Yun Huang; Ufuk Günesdogan; Kristjan A. Gretarsson; Toshihiro Kobayashi; M. Azim Surani

doi:10.1038/s41467-018-06230-0

Nature Communications (Oct 2018)

Tracing the transitions from pluripotency to germ cell fate with CRISPR screening

Jamie A. Hackett,
Yun Huang,
Ufuk Günesdogan,
Kristjan A. Gretarsson,
Toshihiro Kobayashi,
M. Azim Surani

Affiliations

Jamie A. Hackett: Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge
Yun Huang: Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge
Ufuk Günesdogan: Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge
Kristjan A. Gretarsson: Epigenetics and Neurobiology Unit, European Molecular Biology Laboratory (EMBL)
Toshihiro Kobayashi: Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge
M. Azim Surani: Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge

DOI: https://doi.org/10.1038/s41467-018-06230-0
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 12

Abstract

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Primordial Germ Cell-Like Cells (PGCLCs) are an in vitro model for primordial germ cell development. Here, the authors couple a novel compound reporter with CRISPR screening to identify key genes for exit from pluripotency and acquisition of PGCLC fate; specifically identifying Nr5a2 and Zfp296.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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