Rapid Detection of Listeria monocytogenes In Chicken Meat By Real-time PCR without Culture Enrichment

Abstract

Foodborne pathogens can easily contaminate chicken meat due to its high nutritional content, and these pathogens can infect humans. One of the most important pathogens contaminating chicken meat and causing severe public health problems is Listeria monocytogenes, which would be responsible for Listeriosis. Therefore, rapid and sensitive detection of L. monocytogenes in chicken meat samples is of great significance. In the current study, the presence of L. monocytogenes in chicken meat samples collected from several markets in Erzurum was detected by comparing two different DNA isolation methods with the Real-time PCR. As a result of the analyses, it was determined that 34% of the chicken meat samples collected were positive for L. monocytogenes in both two methods. According to the comparison analyses of the Bland-Altman method, no significant difference was found between the thermal lysis method and the DNA isolation method by commercial kit. As a result of this study, it has been shown that the thermal lysis method can be successfully applied for the detection of foodborne pathogens in chicken meat when evaluated in terms of workload and cost. The current study is the first report on the comparison of thermal lysis method and DNA isolation by commercial kit in the detection of L. monocytogenes from chicken meat by Real-time PCR.

Keywords

• chicken meat
• listeria monocytogenes
• public health
• real-time pcr