Derivation of Two New Human Embryonic Stem Cell Lines from Nonviable Human Embryos

Svetlana Gavrilov; Darja Marolt; Nataki C. Douglas; Robert W. Prosser; Imran Khalid; Mark V. Sauer; Donald W. Landry; Gordana Vunjak-Novakovic; Virginia E. Papaioannou

doi:10.4061/2011/765378

Stem Cells International (Jan 2011)

Derivation of Two New Human Embryonic Stem Cell Lines from Nonviable Human Embryos

Svetlana Gavrilov,
Darja Marolt,
Nataki C. Douglas,
Robert W. Prosser,
Imran Khalid,
Mark V. Sauer,
Donald W. Landry,
Gordana Vunjak-Novakovic,
Virginia E. Papaioannou

Affiliations

Svetlana Gavrilov: Department of Genetics and Development, College of Physicians and Surgeons of Columbia University, 701 West 168th Street, New York, NY 10032, USA
Darja Marolt: Department of Biomedical Engineering, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA
Nataki C. Douglas: Department of Obstetrics and Gynecology, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA
Robert W. Prosser: Department of Obstetrics and Gynecology, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA
Imran Khalid: Division of Experimental Therapeutics, Department of Medicine, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA
Mark V. Sauer: Department of Obstetrics and Gynecology, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA
Donald W. Landry: Division of Experimental Therapeutics, Department of Medicine, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA
Gordana Vunjak-Novakovic: Department of Biomedical Engineering, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA
Virginia E. Papaioannou: Department of Genetics and Development, College of Physicians and Surgeons of Columbia University, 701 West 168th Street, New York, NY 10032, USA

DOI: https://doi.org/10.4061/2011/765378
Journal volume & issue: Vol. 2011

Abstract

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We report the derivation and characterization of two new human embryonic stem cells (hESC) lines (CU1 and CU2) from embryos with an irreversible loss of integrated organismic function. In addition, we analyzed retrospective data of morphological progression from embryonic day (ED) 5 to ED6 for 2480 embryos not suitable for clinical use to assess grading criteria indicative of loss of viability on ED5. Our analysis indicated that a large proportion of in vitro fertilization (IVF) embryos not suitable for clinical use could be used for hESC derivation. Based on these combined findings, we propose that criteria commonly used in IVF clinics to determine optimal embryos for uterine transfer can be employed to predict the potential for hESC derivation from poor quality embryos without the destruction of vital human embryos.

Published in Stem Cells International

ISSN: 1687-966X (Print); 1687-9678 (Online)
Publisher: Hindawi Limited
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine
Website: https://onlinelibrary.wiley.com/journal/4162

About the journal