MicroED: conception, practice and future opportunities

Max T. B. Clabbers; Anna Shiriaeva; Tamir Gonen

doi:10.1107/S2052252521013063

IUCrJ (Mar 2022)

MicroED: conception, practice and future opportunities

Max T. B. Clabbers,
Anna Shiriaeva,
Tamir Gonen

Affiliations

Max T. B. Clabbers: Department of Biological Chemistry, University of California, Los Angeles, CA 90095, USA
Anna Shiriaeva: Department of Biological Chemistry, University of California, Los Angeles, CA 90095, USA
Tamir Gonen: Department of Biological Chemistry, University of California, Los Angeles, CA 90095, USA

DOI: https://doi.org/10.1107/S2052252521013063
Journal volume & issue: Vol. 9, no. 2
pp. 169 – 179

Abstract

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This article documents a keynote seminar presented at the IUCr Congress in Prague, 2021. The cryo-EM method microcrystal electron diffraction is described and put in the context of macromolecular electron crystallography from its origins in 2D crystals of membrane proteins to today's application to 3D crystals a millionth the size of that needed for X-ray crystallography. Milestones in method development and applications are described with an outlook to the future.

Published in IUCrJ

ISSN: 2052-2525 (Online)
Publisher: International Union of Crystallography
Country of publisher: United Kingdom
LCC subjects: Science: Chemistry: Crystallography
Website: https://journals.iucr.org/m/

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Abstract

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