Life (Dec 2023)

Fluorescence Confocal Microscopy in Urological Malignancies: Current Applications and Future Perspectives

  • Luca Ongaro,
  • Giulio Rossin,
  • Arianna Biasatti,
  • Matteo Pacini,
  • Michele Rizzo,
  • Fabio Traunero,
  • Andrea Piasentin,
  • Alessandro Perotti,
  • Carlo Trombetta,
  • Riccardo Bartoletti,
  • Alessandro Zucchi,
  • Alchiede Simonato,
  • Nicola Pavan,
  • Giovanni Liguori,
  • Francesco Claps

DOI
https://doi.org/10.3390/life13122301
Journal volume & issue
Vol. 13, no. 12
p. 2301

Abstract

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Fluorescence confocal microscopy (FCM) represents a novel diagnostic technique able to provide real-time histological images from non-fixed specimens. As a consequence of its recent developments, FCM is gaining growing popularity in urological practice. Nevertheless, evidence is still sparse, and, at the moment, its applications are heterogeneous. We performed a narrative review of the current literature on this topic. Papers were selected from the Pubmed, Embase, and Medline archives. We focused on FCM applications in prostate cancer (PCa), urothelial carcinoma (UC), and renal cell carcinoma (RCC). Articles investigating both office and intraoperative settings were included. The review of the literature showed that FCM displays promising accuracy as compared to conventional histopathology. These results represent significant steps along the path of FCM’s formal validation as an innovative ready-to-use diagnostic support in urological practice. Instant access to a reliable histological evaluation may indeed significantly influence physicians’ decision-making process. In this regard, FCM addresses this still unmet clinical need and introduces intriguing perspectives into future diagnostic pathways. Further studies are required to thoroughly assess the whole potential of this technique.

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