Micro Fragmented Adipose Tissue Promotes the Matrix Synthesis Function of Nucleus Pulposus Cells and Regenerates Degenerated Intervertebral Disc in a Pig Model

Xiaopeng Zhou; Feng Zhang; Dawei Wang; Jingkai Wang; Chenggui Wang; Kaishun Xia; Liwei Ying; Xianpeng Huang; Yiqing Tao; Shouyong Chen; Deting Xue; Jianming Hua; Chengzhen Liang; Qixin Chen; Fangcai Li

doi:10.1177/0963689720905798

Cell Transplantation (Feb 2020)

Micro Fragmented Adipose Tissue Promotes the Matrix Synthesis Function of Nucleus Pulposus Cells and Regenerates Degenerated Intervertebral Disc in a Pig Model

Xiaopeng Zhou,
Feng Zhang,
Dawei Wang,
Jingkai Wang,
Chenggui Wang,
Kaishun Xia,
Liwei Ying,
Xianpeng Huang,
Yiqing Tao,
Shouyong Chen,
Deting Xue,
Jianming Hua,
Chengzhen Liang,
Qixin Chen,
Fangcai Li

Affiliations

Xiaopeng Zhou: These authors contributed equally to this article.
Feng Zhang: These authors contributed equally to this article.
Dawei Wang: These authors contributed equally to this article.
Jingkai Wang: These authors contributed equally to this article.
Chenggui Wang: Department of Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China
Kaishun Xia: Department of Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China
Liwei Ying: Department of Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China
Xianpeng Huang: Department of Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China
Yiqing Tao: Department of Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China
Shouyong Chen: Department of Orthopedics Surgery, The Affiliated Hospital of Hangzhou Normal University, Hangzhou, Zhejiang, People’s Republic of China
Deting Xue: Department of Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China
Jianming Hua: Department of Radiology, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, People’s Republic of China
Chengzhen Liang: Department of Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China
Qixin Chen: Department of Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China
Fangcai Li: Department of Orthopedics Research Institute of Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China

DOI: https://doi.org/10.1177/0963689720905798
Journal volume & issue: Vol. 29

Abstract

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Intervertebral disc (IVD) degeneration and consequent lower back pain is a common disease. Micro fragmented adipose tissue (MFAT) is promising for a wide range of applications in regenerative medicine. In this study, MFAT was isolated by a nonenzymatic method and co-cultured with nucleus pulposus cells (NPCs) using an indirect co-culture system in vitro . A pig disc degeneration model was used to investigate the regenerative effect of MFAT on degenerated IVDs in vivo . The mRNA expression of Sox9 , Acan , and Col2 in NPCs was significantly increased, while no significant increase was observed in the mRNA expression of proinflammatory cytokine genes after the NPCs were co-cultured with MFAT. Nucleus pulposus (NP)-specific markers were increased in MFAT cells after co-culture with NPCs. After injection of MFAT, the disc height, water content, extracellular matrix, and structure of the degenerated NP were significantly improved. MFAT promoted the matrix synthesis function of NPCs, and NPCs stimulated the NP-like differentiation of MFAT cells. In addition, MFAT also partly regenerated degenerated IVDs in the pig model.

Published in Cell Transplantation

ISSN: 0963-6897 (Print); 1555-3892 (Online)
Publisher: SAGE Publishing
Country of publisher: United States
LCC subjects: Medicine
Website: http://journals.sagepub.com/home/cll

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