The effect’s evaluation of biological products on the morphometric and physiological parameters of plants-remediants in conditions of soil oil pollution

Abstract

Background. Plants are tolerant to numerous environmental pollutants and are often used in phytoremediation strategies, but high pollutant concentrations disrupt the growth and development of the plants themselves. In this regard, it seems important to use additional methods for increasing the resistance of plants to the action of pollutants. One of these methods is the use of biological products based on hydrocarbon-oxidizing micro-organisms, which combine the ability to destroy oil and stimulate plant growth in the same bacterial culture. The purpose of this study was a comparative assessment of the effect of biological products on the growth rates of phytoremediated plants under conditions of soil oil pollution. Materials and methods. Oil was added to the gray forest soil at a concentra-tion of 4 % of the dry mass of the soil. After the uniform distribution of the pollutant in the soil, biopreparations “Lenoil”, “Elena” and “Azolene” were introduced into it, and then alfalfa and rye seeds were planted. A comparative assessment of the effect of bioprepara-tions, as well as complexes of drugs “Lenoil + Elena” and “Lenoil + Azolene” on the mor-phometric parameters of plants was carried out. Results and conclusions. Biopreparation “Elena” showed the greatest growth-stimulating effect on plants of alfalfa and rye in condi-tions of oil pollution at a concentration of 4 %. To a lesser extent, the growth performance of plants was influenced by the preparations “Lenoil” and “Azolene”. The use of biological products led to an increase in the content of chlorophyll a and b, carotenoids, and the activi-ty of the enzyme catalase. The species specificity of the action of biological products on physiological indicators was established, so when using the biological product “Elena”, the concentration of chlorophylls a, b and carotenoids was higher in alfalfa, a representative of the family – Legumes, and when using the biological product “Azolene”, indicators were better in rye, which belongs to the Cereals family. The use of biological products “Elena” and “Azolene” after using the drug “Lenoil” led to a decrease in the activity of growth pro-cesses in the studied plants.

Keywords

• phytoremediation
• soil oil pollution
• biological products
• chlorophyll and caro-tenoid content
• catalase activity