Diabetes, Metabolic Syndrome and Obesity (Jan 2023)
Investigation of the Correlation Between the Polymorphism/Expression Level of RANTES and Its Receptor CCR5 Gene Promoter and Type 2 Diabetes Mellitus
Abstract
Jie Chen,1,* Weichang Guo,2,* Hejia Yin,1 Liju Ma,3 Shaoyou Li,4 Huifang Li1 1Department of Diabetes, The First Affiliated Hospital of Kunming Medical University, Kunming, 650032, People’s Republic of China; 2Department of Physical Education, Kunming Medical University, Kunming, 650032, People’s Republic of China; 3Department of Clinical Laboratory, The First Affiliated Hospital of Kunming Medical University, Kunming, 650032, People’s Republic of China; 4Department of The Scientific Research Laboratory Center, The First Affiliated Hospital of Kunming Medical University, Kunming, 650032, People’s Republic of China*These authors contributed equally to this workCorrespondence: Huifang Li, Department of Diabetes, The First Affiliated Hospital of Kunming Medical University, No. 295 Xichang Road, Wuhua District, Kunming, 650032, People’s Republic of China, Email [email protected]: This study aimed to explore relationship among RANTES − 28 (rs2280788) C/G polymorphism or CCR5 59029 (rs1799987) A/G polymorphism, level of self-expression, and type 2 diabetes mellitus (T2DM).Materials and Methods: Clinical data were collected from 92 subjects with normal blood glucose (NC) and 97 patients with T2DM (DM). CCR5 levels on the surface of monocyte/lymphocyte and plasma RANTES levels were detected by flow cytometry. TaqMan real-time fluorescent quantitative PCR was used to detect genetic polymorphisms of RANTES rs2280788 and CCR5 rs1799987.Results: There were no significant differences in frequencies of CCR5 rs1799987 genotype and A/G allele and frequencies of RANTES rs2280788 genotype and C/G allele, between subjects in NC and DM group (P > 0.05). Plasma RANTES level in DM group was significantly lower than NC group (P 0.05). RANTES level was positively correlated with age and TC and negatively correlated with diabetes course and HbA1c. CCR5 level on the surface of monocytes was positively correlated with drinking years, HbA1c, course of diabetes, and negatively correlated with TC. CCR5 on lymphocyte surface was positively correlated with diabetes course, smoking years, HbA1c, and negatively correlated with LDL, TC, HDL (P < 0.05).Conclusion: RANTES -28 (rs2280788) C/G polymorphism or CCR5 59029 (rs1799987) A/G polymorphism may not be associated with T2DM of Han nationality in Kunming and cannot affect RANTES and CCR5 expression. RANTES and CCR5 levels may be related to T2DM but may also be affected by age, blood lipids, HbA1c, diabetes course, drugs, and other factors.Keywords: type 2 diabetes, inflammation, cytokines, gene polymorphism, RANTES, CCR5
