Communications Biology (May 2021)
DNA methylation changes during long-term in vitro cell culture are caused by epigenetic drift
- Julia Franzen,
- Theodoros Georgomanolis,
- Anton Selich,
- Chao-Chung Kuo,
- Reinhard Stöger,
- Lilija Brant,
- Melita Sara Mulabdić,
- Eduardo Fernandez-Rebollo,
- Clara Grezella,
- Alina Ostrowska,
- Matthias Begemann,
- Miloš Nikolić,
- Björn Rath,
- Anthony D. Ho,
- Michael Rothe,
- Axel Schambach,
- Argyris Papantonis,
- Wolfgang Wagner
Affiliations
- Julia Franzen
- Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University Medical School
- Theodoros Georgomanolis
- Center for Molecular Medicine, University of Cologne
- Anton Selich
- Institute of Experimental Hematology, Hannover Medical School
- Chao-Chung Kuo
- Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University Medical School
- Reinhard Stöger
- School of Biosciences, University of Nottingham, Sutton Bonington Campus
- Lilija Brant
- Center for Molecular Medicine, University of Cologne
- Melita Sara Mulabdić
- Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University Medical School
- Eduardo Fernandez-Rebollo
- Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University Medical School
- Clara Grezella
- Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University Medical School
- Alina Ostrowska
- Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University Medical School
- Matthias Begemann
- Institute of Human Genetics, Medical Faculty, RWTH Aachen University
- Miloš Nikolić
- Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University Medical School
- Björn Rath
- Department for Orthopedics, RWTH Aachen University Medical School
- Anthony D. Ho
- Internal Medicine Department of Hematology, Oncology and Rheumatology, Heidelberg University Medical Center
- Michael Rothe
- Institute of Experimental Hematology, Hannover Medical School
- Axel Schambach
- Institute of Experimental Hematology, Hannover Medical School
- Argyris Papantonis
- Center for Molecular Medicine, University of Cologne
- Wolfgang Wagner
- Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University Medical School
- DOI
- https://doi.org/10.1038/s42003-021-02116-y
- Journal volume & issue
-
Vol. 4,
no. 1
pp. 1 – 12
Abstract
Julia Franzen et al. investigate if changes in DNA methylation at specific genetic loci during cell culture expansion are due to a specific mechanism or gradual deregulation of an epigenetic state. Their results suggest that changes in CpG methylation are due to indirect epigenetic drift, rather than a consequence of targeting by DNA methyltransferases.
