Isolation of myeloid cells from mouse brain tumors for single-cell RNA-seq analysis

Abstract

Summary: Current single-cell RNA sequencing (scRNA-seq) protocols are limited by the number of cells that can be simultaneously sequenced, restricting the ability to resolve heterogeneity of rare cell types. We describe here a protocol for rapid isolation of myeloid cells from tumor-harboring mouse cerebellum without cell sorting to minimize cell damage for scRNA-seq. This protocol includes the procedures for further enrichment of myeloid cells using CD11b+ magnetic beads, followed by the generation of scRNA library and sequencing analysis.For complete details on the use and execution of this protocol, please refer to Dang et al. (2021).

Keywords

• Cell Biology
• Cell isolation
• Single Cell
• Cancer
• Sequencing
• RNAseq