The GDNF-gel/HA-Mg conduit promotes the repair of peripheral nerve defects by regulating PPAR-γ/RhoA/ROCK signaling pathway

Yuanqing Cai; Yi Chen; Guangyang Zhang; Yi Lin; Jianan Zhang; Jialin Liang; Leifeng Lv; Yong Wang; Xinyu Fang; Xiaoqian Dang

iScience (Feb 2024)

The GDNF-gel/HA-Mg conduit promotes the repair of peripheral nerve defects by regulating PPAR-γ/RhoA/ROCK signaling pathway

Yuanqing Cai,
Yi Chen,
Guangyang Zhang,
Yi Lin,
Jianan Zhang,
Jialin Liang,
Leifeng Lv,
Yong Wang,
Xinyu Fang,
Xiaoqian Dang

Affiliations

Yuanqing Cai: Department of Orthopaedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710006, China
Yi Chen: College of Materials Science & Engineering, National Engineering Research Center for Magnesium Alloys, Chongqing University, Chongqing 400045, China
Guangyang Zhang: Department of Orthopaedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710006, China
Yi Lin: Department of Ophthalmology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing 401120, China
Jianan Zhang: Zonglian College, Xi’an Jiaotong University, Xi’an 710054, China
Jialin Liang: Department of Orthopaedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710006, China
Leifeng Lv: Department of Orthopaedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710006, China
Yong Wang: College of Materials Science & Engineering, National Engineering Research Center for Magnesium Alloys, Chongqing University, Chongqing 400045, China; Corresponding author
Xinyu Fang: Department of Orthopaedic Surgery, the First Affiliated Hospital, Fujian Medical University, Fuzhou 350005, China; Corresponding author
Xiaoqian Dang: Department of Orthopaedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710006, China; Corresponding author

Journal volume & issue: Vol. 27, no. 2
p. 108969

Abstract

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Summary: Magnesium (Mg)-based conduits have gained more attention in repairing peripheral nerve defects. However, they are limited due to poor corrosion resistance and rapid degradation rate. To tackle this issue, glial cell line-derived neurotrophic factor (GDNF)- Gelatin methacryloyl (Gel)/hydroxylapatite (HA)-Mg nerve conduit was developed and implanted in sciatic nerve defect model in Sprague-Dawley (SD) rats. The sciatic functional index measurement showed that the GDNF-Gel/HA-Mg nerve conduit effectively promoted the recovery of sciatic nerve function. The pathological examination results showed that there were more regenerated nerve tissues in GDNF-Gel/HA-Mg group, with a higher number of regenerating axons, and the thickness of the myelin sheath was significantly larger than that of control group (NC group). Immunofluorescence results revealed that the GDNF-Gel/HA-Mg conduit significantly promoted the expression of genes associated with nerve repair. RNA-seq and molecular test results indicated that GDNF-Gel/HA-Mg might be involved in the repair of peripheral nerve defects by regulating PPAR-γ/RhoA/ROCK signaling pathway.Biological sciences; Neuroscience; Molecular neuroscience; Techniques in neuroscience

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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