Nature Communications (Aug 2019)
Pluripotency reprogramming by competent and incompetent POU factors uncovers temporal dependency for Oct4 and Sox2
- Vikas Malik,
- Laura V. Glaser,
- Dennis Zimmer,
- Sergiy Velychko,
- Mingxi Weng,
- Markus Holzner,
- Marius Arend,
- Yanpu Chen,
- Yogesh Srivastava,
- Veeramohan Veerapandian,
- Zahir Shah,
- Miguel A. Esteban,
- Huating Wang,
- Jiekai Chen,
- Hans R. Schöler,
- Andrew P. Hutchins,
- Sebastiaan H. Meijsing,
- Sebastian Pott,
- Ralf Jauch
Affiliations
- Vikas Malik
- CAS Key Laboratory of Regenerative Biology, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical University
- Laura V. Glaser
- Max Planck Institute for Molecular Genetics
- Dennis Zimmer
- CAS Key Laboratory of Regenerative Biology, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical University
- Sergiy Velychko
- Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine
- Mingxi Weng
- School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong
- Markus Holzner
- School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong
- Marius Arend
- School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong
- Yanpu Chen
- CAS Key Laboratory of Regenerative Biology, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical University
- Yogesh Srivastava
- CAS Key Laboratory of Regenerative Biology, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical University
- Veeramohan Veerapandian
- CAS Key Laboratory of Regenerative Biology, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical University
- Zahir Shah
- University of Chinese Academy of Sciences
- Miguel A. Esteban
- CAS Key Laboratory of Regenerative Biology, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical University
- Huating Wang
- Department of Orthopaedics and Traumatology, Prince of Wales Hospital, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong
- Jiekai Chen
- CAS Key Laboratory of Regenerative Biology, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical University
- Hans R. Schöler
- Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine
- Andrew P. Hutchins
- Department of Biology, Southern University of Science and Technology
- Sebastiaan H. Meijsing
- Max Planck Institute for Molecular Genetics
- Sebastian Pott
- Department of Human Genetics, The University of Chicago
- Ralf Jauch
- CAS Key Laboratory of Regenerative Biology, Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical University
- DOI
- https://doi.org/10.1038/s41467-019-11054-7
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 16
Abstract
Oct4, along with Sox2 and Klf4 can induce pluripotency, but structurally similar factors like Oct6 cannot. Here, using pluripotency competent and incompetent factors, the authors show that Sox2 plays a dominant role in facilitating chromatin opening at Oct4 bound DNA early during reprogramming to pluripotency.
