Fibrochondrogenic differentiation potential of tendon-derived stem/progenitor cells from human patellar tendon

Shengnan Qin; Wen Wang; Zhihe Liu; Xing Hua; SaiChuen Fu; Fei Dong; Aiguo Li; Zhen Liu; Pengzhen Wang; Libing Dai; Peihong Liang; Jinli Zhang; Wenjuan Cao; Xifeng Xiong; Honghui Chen; Jiake Xu

Journal of Orthopaedic Translation (May 2020)

Fibrochondrogenic differentiation potential of tendon-derived stem/progenitor cells from human patellar tendon

Shengnan Qin,
Wen Wang,
Zhihe Liu,
Xing Hua,
SaiChuen Fu,
Fei Dong,
Aiguo Li,
Zhen Liu,
Pengzhen Wang,
Libing Dai,
Peihong Liang,
Jinli Zhang,
Wenjuan Cao,
Xifeng Xiong,
Honghui Chen,
Jiake Xu

Affiliations

Shengnan Qin: Guangzhou Institute of Traumatic Surgery, Department of Orthopaedics, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, China
Wen Wang: Guangzhou Institute of Traumatic Surgery, Department of Orthopaedics, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, China; Corresponding author. Department of pathology, Guangzhou Red Cross Hospital, Medical College, Jinan University, NO.396, Rd. Tongfu, Guangzhou, 510220, China.
Zhihe Liu: Guangzhou Institute of Traumatic Surgery, Department of Orthopaedics, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, China
Xing Hua: Department of Pathology, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, China
SaiChuen Fu: Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong
Fei Dong: Guangzhou Institute of Traumatic Surgery, Department of Orthopaedics, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, China
Aiguo Li: Guangzhou Institute of Traumatic Surgery, Department of Orthopaedics, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, China
Zhen Liu: Department of Pathology, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, China
Pengzhen Wang: Guangzhou Institute of Traumatic Surgery, Department of Orthopaedics, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, China
Libing Dai: Guangzhou Institute of Traumatic Surgery, Department of Orthopaedics, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, China
Peihong Liang: Guangzhou Institute of Traumatic Surgery, Department of Orthopaedics, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, China
Jinli Zhang: Guangzhou Institute of Traumatic Surgery, Department of Orthopaedics, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, China
Wenjuan Cao: Guangzhou Institute of Traumatic Surgery, Department of Orthopaedics, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, China
Xifeng Xiong: Guangzhou Institute of Traumatic Surgery, Department of Orthopaedics, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, China
Honghui Chen: Guangzhou Institute of Traumatic Surgery, Department of Orthopaedics, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, China
Jiake Xu: School of Biomedical Sciences, The University of Western Australia, Perth, Australia; Corresponding author. School of Biomedical Sciences, The University of Western Australia, 35 Stirling Highway Perth WA 6009, Australia.

Journal volume & issue: Vol. 22
pp. 101 – 108

Abstract

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Background: Bone–tendon junction (BTJ) is a unique structure connecting tendon and bone through a fibrocartilage zone. Owing to its unique structure, the regeneration of BTJ remains a challenge. Here, we study the fibrochondrogenic differentiation of human tendon-derived stem/progenitor cells (TSPCs) both in vitro and in vivo. Methods: TSPCs were isolated from human patellar tendon tissues and investigated for their multidifferentiation potential. TSPCs were cultured in chondrogenic medium with transforming growth factor beta 3 (TGF-β3) and BMP-2 in vitro and examined for the expression of fibrochondrogenic marker genes by quantitative real-time reverse transcription polymerase chain reaction, enzyme-linked immunosorbent assay, and immunofluorescence. TSPCs pretreated were also seeded in collage II sponge and then transplanted in immunocompromised nude mice to examine if the fibrochondrogenic characteristics were conserved in vivo. Results: We found that TSPCs were differentiated towards fibrochondrogenic lineage, accompanied by the expression of collagen I, collagen II, SRY-box transcription factor 9 (Sox 9), and tenascin C. Furthermore, after TSPCs were seeded in collagen II sponge and transplanted in immunocompromised nude mice, they expressed fibrochondrogenic genes, including proteoglycan, collagen I, and collagen II. Conclusion: Taken together, this study showed that TSPCs are capable of differentiating towards fibrocartilage-like cells, and the fibrochondrogenic characteristics were conserved even in vivo, and thus might have the potential application for fibrocartilage regeneration in BTJ repair. The translational potential of this article: TSPCs are able to differentiate into fibrocartilage-like cells and thus might well be one potential cell source for fibrocartilage regeneration in a damaged BTJ repair.

Published in Journal of Orthopaedic Translation

ISSN: 2214-031X (Print); 2214-0328 (Online)
Publisher: Elsevier
Country of publisher: Hong Kong
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Diseases of the musculoskeletal system
Website: http://www.journals.elsevier.com/journal-of-orthopaedic-translation/

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