Structural insights into inhibitory mechanism of human excitatory amino acid transporter EAAT2

Takafumi Kato; Tsukasa Kusakizako; Chunhuan Jin; Xinyu Zhou; Ryuichi Ohgaki; LiLi Quan; Minhui Xu; Suguru Okuda; Kan Kobayashi; Keitaro Yamashita; Tomohiro Nishizawa; Yoshikatsu Kanai; Osamu Nureki

doi:10.1038/s41467-022-32442-6

Nature Communications (Aug 2022)

Structural insights into inhibitory mechanism of human excitatory amino acid transporter EAAT2

Takafumi Kato,
Tsukasa Kusakizako,
Chunhuan Jin,
Xinyu Zhou,
Ryuichi Ohgaki,
LiLi Quan,
Minhui Xu,
Suguru Okuda,
Kan Kobayashi,
Keitaro Yamashita,
Tomohiro Nishizawa,
Yoshikatsu Kanai,
Osamu Nureki

Affiliations

Takafumi Kato: Department of Biological Science, Graduate School of Science, The University of Tokyo
Tsukasa Kusakizako: Department of Biological Science, Graduate School of Science, The University of Tokyo
Chunhuan Jin: Department of Bio-system Pharmacology, Graduate School of Medicine, Osaka University
Xinyu Zhou: Department of Bio-system Pharmacology, Graduate School of Medicine, Osaka University
Ryuichi Ohgaki: Department of Bio-system Pharmacology, Graduate School of Medicine, Osaka University
LiLi Quan: Department of Bio-system Pharmacology, Graduate School of Medicine, Osaka University
Minhui Xu: Department of Bio-system Pharmacology, Graduate School of Medicine, Osaka University
Suguru Okuda: Department of Bio-system Pharmacology, Graduate School of Medicine, Osaka University
Kan Kobayashi: Department of Biological Science, Graduate School of Science, The University of Tokyo
Keitaro Yamashita: Department of Biological Science, Graduate School of Science, The University of Tokyo
Tomohiro Nishizawa: Department of Biological Science, Graduate School of Science, The University of Tokyo
Yoshikatsu Kanai: Department of Bio-system Pharmacology, Graduate School of Medicine, Osaka University
Osamu Nureki: Department of Biological Science, Graduate School of Science, The University of Tokyo

DOI: https://doi.org/10.1038/s41467-022-32442-6
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 11

Abstract

Read online

EAAT2 is an amino acid transporter implicated in glutamate homeostasis in brain and therapy resistance of cancer cells. Here, the authors report cryo-EM structures and reveal inhibitory mechanisms via selective inhibitor WAY213613.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal