MicroRNAs Differentially Expressed in Actinic Keratosis and Healthy Skin Scrapings

Maria Vincenza Chiantore; Marco Iuliano; Roberta Maria Mongiovì; Fabiola Luzi; Giorgio Mangino; Lorenzo Grimaldi; Luisa Accardi; Gianna Fiorucci; Giovanna Romeo; Paola Di Bonito

doi:10.3390/biomedicines11061719

Biomedicines (Jun 2023)

MicroRNAs Differentially Expressed in Actinic Keratosis and Healthy Skin Scrapings

Maria Vincenza Chiantore,
Marco Iuliano,
Roberta Maria Mongiovì,
Fabiola Luzi,
Giorgio Mangino,
Lorenzo Grimaldi,
Luisa Accardi,
Gianna Fiorucci,
Giovanna Romeo,
Paola Di Bonito

Affiliations

Maria Vincenza Chiantore: EVOR Unit, Department of Infectious Diseases, Istituto Superiore di Sanità, 00161 Rome, Italy
Marco Iuliano: Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome—Polo Pontino, 04100 Latina, Italy
Roberta Maria Mongiovì: Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome—Polo Pontino, 04100 Latina, Italy
Fabiola Luzi: Plastic and Reconstructive Surgery, San Gallicano Dermatologic Institute IRCCS, 00144 Rome, Italy
Giorgio Mangino: Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome—Polo Pontino, 04100 Latina, Italy
Lorenzo Grimaldi: Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome—Polo Pontino, 04100 Latina, Italy
Luisa Accardi: EVOR Unit, Department of Infectious Diseases, Istituto Superiore di Sanità, 00161 Rome, Italy
Gianna Fiorucci: EVOR Unit, Department of Infectious Diseases, Istituto Superiore di Sanità, 00161 Rome, Italy
Giovanna Romeo: Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome—Polo Pontino, 04100 Latina, Italy
Paola Di Bonito: EVOR Unit, Department of Infectious Diseases, Istituto Superiore di Sanità, 00161 Rome, Italy

DOI: https://doi.org/10.3390/biomedicines11061719
Journal volume & issue: Vol. 11, no. 6
p. 1719

Abstract

Read online

Actinic keratosis (AK) is a carcinoma in situ precursor of cutaneous squamous cell carcinoma (cSCC), the second most common cancer affecting the Caucasian population. AK is frequently present in the sun-exposed skin of the elderly population, UV radiation being the main cause of this cancer, and other risk factors contributing to AK incidence. The dysregulation of microRNAs (miRNAs) observed in different cancers leads to an improper expression of miRNA targets involved in several cellular pathways. The TaqMan Array Human MicroRNA Card assay for miRNA expression profiling was performed in pooled AK compared to healthy skin scraping samples from the same patients. Forty-three miRNAs were modulated in the AK samples. The expression of miR-19b (p p p p −10 p −8). Selected genes regulating the KEGG pathways, i.e., TP53, MDM2, CDKN1A, CDK6, and CCND1, were analyzed. MiRNAs modulated in AK regulate different pathways involved in tumorigenesis, indicating miRNA regulation as a critical step in keratinocyte cancer.

Published in Biomedicines

ISSN: 2227-9059 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: http://www.mdpi.com/journal/biomedicines

About the journal

Abstract

Keywords